{"id":41036,"date":"2023-02-18T17:00:13","date_gmt":"2023-02-18T18:00:13","guid":{"rendered":"https:\/\/peymantaeidi.net\/stem-cell\/?p=41036"},"modified":"2023-02-18T18:37:11","modified_gmt":"2023-02-18T18:37:11","slug":"a-multifunctional-highly-biocompatible-and-double-triggering-caramelized-nanotheranostic-system-loaded-with-fe3o4-and-dox-for-combined-chemo-photothermal-therapy-and-real-time-magnetic-resonance-ima","status":"publish","type":"post","link":"https:\/\/peymantaeidi.net\/stem-cell\/2023\/02\/18\/a-multifunctional-highly-biocompatible-and-double-triggering-caramelized-nanotheranostic-system-loaded-with-fe3o4-and-dox-for-combined-chemo-photothermal-therapy-and-real-time-magnetic-resonance-ima\/","title":{"rendered":"A Multifunctional, Highly Biocompatible, and Double-Triggering Caramelized Nanotheranostic System Loaded with Fe3O4 and DOX for Combined Chemo-Photothermal Therapy and Real-Time Magnetic Resonance Imaging Monitoring of Triple Negative Breast Cancer"},"content":{"rendered":"
\n

Introduction<\/h2>\n

Breast cancer is one of the commonly diagnosed cancer in the world and the disease is the leading cause of cancer mortality in women worldwide.1<\/a><\/sup> The triple negative breast cancer (TNBC) subtype is defined by the lack of expression of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2.2<\/a><\/sup> Clinical management of TNBC is a great challenge because of its strong invasiveness, large heterogeneity, and high malignancy.3<\/a>,4<\/a><\/sup> Except for surgical resection for early-stage tumors,5<\/a>,6<\/a><\/sup> other methods including radiotherapy, chemotherapy, endocrine and targeted therapy are usually limited by poor targeting, multidrug resistance (MDR), and side effects that cause serious damage to non-cancerous tissues.7\u201310<\/a><\/sup> At present, combination therapy is the main route to improve the curative effect of TNBC; however, the overall survival rate is still not high, and the side effects are cumulative.11<\/a><\/sup> In the diagnosis and assessment of the treatment efficacy, non-invasive imaging methods including ultrasound, mammography, computed tomography (CT) and magnetic resonance imaging (MRI).12<\/a><\/sup> Among these, MRI is readily available, non-invasive, does not rely on the use of ionizing radiation, has high spatial resolution, soft tissue and spatial resolution.13<\/a><\/sup> However, the common MRI contrast agent currently used in clinical practice is non-specific with neurotoxicity and nephrotoxicity.14<\/a><\/sup> Therefore, a targeted and safe MRI contrast agent is emerging as a major clinical requirement for imaging diagnosis and monitoring.<\/p>\n

With the development of nanomedicine in the last few decades, the multifunctional and multimodal theragnostic nano-platform has shown immense potential in combining treatment and diagnosis with synergetic reinforcing effects for malignant tumors in recent years and can aid in overcoming the challenges with the diagnosis and treatment of TNBC. However, some problems remain, such as the nanocarrier toxicity, complicated combination modalities, and interference from different components, which may limit its clinical translation and application.15\u201317<\/a><\/sup><\/p>\n

In recent years, photothermal therapy (PTT) has emerged as a novel cancer treatment that uses photothermal therapy agents (PTAs) to absorb near-infrared (NIR) light energy and convert this into heat energy, causing local overheating to thermally ablate target cells while causing minimal damage to the surrounding normal cells and tissues.18<\/a>,19<\/a><\/sup> Moreover, PTT-derived multimodal combination treatments have received extensive attention and exhibited cooperatively enhanced anticancer effects. Some new nanomaterials such as those based on gold, carbon, and manganese have excellent NIR absorption efficiency, and could be considered as candidate materials for PTAs.20\u201322<\/a><\/sup> However, the unknown toxicity and biocompatibility hinder their use in long-term clinical studies.23<\/a><\/sup> Our previous studies have shown that food-grade caramelized nanospheres (CNSs) are safe carriers in biological applications with high biocompatibility and low toxicity,24<\/a><\/sup> and their robust \u03c0-\u03c0 conjugated structure could act as excellent NIR light absorbing agents for PTT.25<\/a><\/sup> The rich functional groups (hydroxyl and carboxyl groups) on their surface can interact easily with PTAs and antitumor drugs.24<\/a>,26<\/a><\/sup> In addition, superparamagnetic Fe3<\/sub>O4<\/sub> nanoparticles (NPs) with less expensive to produce, good biocompatibility and low toxicity, approved by the USA Food and Drug Administration (FDA),27\u201333<\/a><\/sup> also offer a high photothermal conversion efficiency and T2-relaxation effects for MRI, demonstrating suitable combining theragnostic effects for PTT and real-time MRI monitoring of the treatment.27\u201329<\/a><\/sup> Moreover, CNSs, in combination with PTT and conventional chemotherapy using doxorubicin hydrochloride (DOX), may achieve pH\/NIR sensitive drug release, thereby significantly enhancing local drug enrichment in the tumor and reducing adverse effects at lower drug doses.17<\/a><\/sup><\/p>\n

In the present study, we successfully synthesized a multifunctional nanosystem, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, to realize combination chemo-PTT treatment and real-time MRI monitoring for improved TNBC treatment. In this system, the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> Fe3O4\/[email protected]<\/a> has longer circulation and strong ability to transform incident 808 nm NIR light into heat energy for photothermal drug release; it is also an appealing candidate for future nanomedicine translation due to their accurate MR imaging properties and effective combination with PTT.<\/p>\n

Experimental<\/h2>\n

Materials and Reagents<\/h3>\n

All reagents were commercially available. Ferric trichloride hexahydrate (FeCl3<\/sub>\u00b76H2<\/sub>O), ammonium hydroxide (NH3<\/sub>\u00b7H2<\/sub>O), and D-(+)-glucose were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Ferrous chloride tetrahydrate (FeCl2<\/sub>\u00b74H2<\/sub>O) was obtained from Aladdin Chemical Reagent Co., Ltd (China). Deionized water (18.25 M\u03a9\u00b7cm) was used during the experiment. Phosphate buffered saline (PBS) (pH = 6.5) and DOX were purchased from Beijing solarbio Science and Technology Co., Ltd (China). 1-(3-Dimethylaminopropyl)-3-ethylcar bodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) were obtained from Beijing Fine Chemical Regent Co., Ltd. (China). The 4T1 (Mouse breast cancer) cell line, MCF-10A (Mammary epithelial) cell line and THP-1 (Human mononuclear) cell lines were purchased from icell Bioscience Inc (Shanghai, China).<\/p>\n

Experimental Apparatus<\/h3>\n

The morphology was characterized by transmission electron microscopy (JEM-2100, JEOL, Japan) and scanning electron microscopy (JEM-6700F, JEOL, Japan) with an energy dispersive spectroscope (EDS). The zeta potentials and size distribution of nanomaterials dispersed in water were determined on a Malvern Zetasizer Nano ZS (Malvern Instruments Ltd., Worcestershire, U.K.). The content of Fe was measured by inductively coupled plasma-atomic mass spectrometry (ICP-MS, Becton Dickinson, USA). Absorption spectra were captured by an ultraviolet (UV) spectrophotometer (TU-1901, Puxi General Instrument Co., Ltd. Beijing, China). The X-ray diffraction (XRD) was investigated on an advanced X-ray diffractometer (Bruker D8, Bruker, Germany) with graphite monochromatized Cu K\u03b1 radiation (\u03bb=1.5418 \u00c5). The magnetic properties were determined using a multifunctional vibrating sample magnetometer (VSM, VersalLab) at room temperature. The UV-visible spectra were measured using a U3900 spectrophotometer (Hitachi, Tokyo, Japan). MRI scanning was conducted using a 3.0 T MRI instrument (GE Signa HDX 3.0T, USA). The optical density (OD) of the cells was measured using a multifunctional microplate reader (Molecular Devices Co., USA). Temperature measurements were recorded using an IR thermal camera (S6-a, IRS, China). Cell images were captured using a biological microscope (BX-53, Olympus, Japan). The concentration of DOX in Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> was determined by high performance liquid chromatography (HPLC, Shimadzu Technologies, Kyoto, Japan).<\/p>\n

Synthesis of CNSs<\/h3>\n

CNSs were prepared according to our report.34<\/a><\/sup> Briefly, 4 g D-(+)-glucose was dissolved in 35 mL water and the solution was heated and kept at 180\u00b0C for 6 h. Finally, the brown products were centrifuged (12,000 rpm, 10 min) and the substrate was washed with ethanol and water. The obtained product was dried in vacuum for further use.<\/p>\n

Synthesis of Fe3<\/sub>O4<\/sub><\/h3>\n

Fe3<\/sub>O4<\/sub> NPs were prepared as follows:34<\/a><\/sup> 0.28 mmol of FeCl3<\/sub>\u00b76H2<\/sub>O and 0.20 mmol FeCl2<\/sub>\u00b74H2<\/sub>O were dissolved in 40 mL of deionized water with a stream of N2<\/sub> to remove O2<\/sub>. The mixture was heated and kept at 80\u00b0C for 1 h, following which 3 mL of NH3<\/sub>\u00b7H2<\/sub>O was added dropwise. After cooling to room temperature, the product was separated using a magnet and washed with water until the solution pH was neutral. The final sample was dried for further use.<\/p>\n

Synthesis of Fe3<\/sub>O4<\/sub>@CNSs<\/h3>\n

First, a 100 mL CNS suspension (0.4 mg\/mL) was mixed with a 50 mL Fe3<\/sub>O4<\/sub> solution (0.2 mg\/mL) using ultrasonication for 1 h. Subsequently, 1 mL glutaraldehyde was added, and the solution was stirred for another 6 h at room temperature. Finally, the solution was allowed to separate for 10 min and the upper layer was obtained by magnetic separation. The obtained Fe3<\/sub>O4<\/sub>@CNSs were washed with ethanol and water and dried for further use. The loading of Fe3<\/sub>O4<\/sub> was calculated using equation:<\/p>\n

\"image\"<\/p>\n

where m0 is the Fe detected by ICP-AES and m1 is the amount of CNSs injected.<\/p>\n

Synthesis of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

10 mg Fe3<\/sub>O4<\/sub>@CNSs (Fe content of 660 mg\/g) was dispersed in 50 mL of PBS under ultrasonication, and 10 mg DOX was added under vigorous stirring for 12 h. The product was separated by a magnet and washed with water. Finally, the dried Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were stored at 4\u00b0C in the dark for further use. The loading of DOX was calculated using equation:<\/p>\n

\"image\"<\/p>\n

where m2 is the total drug injected, m3 is drug in supernatants and m4 is Fe3<\/sub>O4<\/sub>@CNSs injected.<\/p>\n

2.7. pH\/NIR Triggered Drug Release<\/h3>\n

The Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were dispersed in 2 mL of pH = 7.4 or 5.0 PBS, and then shaken gently for pH-responsive drug release assay. The solution was centrifuged for a predetermined time, the original PBS was replaced with 2 mL of fresh PBS, and the UV-vis absorption of PBS was measured at a wavelength of 480 nm. Likewise, an 808 nm laser detector was installed 3 cm from the tube core in which the PBS solution was Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (pH = 7.4 or 5.0). Specimens were irradiated with 808 nm NIR light for 5 min and then centrifuged at 7000 rpm for 4 min. Quantification of released free DOX by UV-vis spectroscopy at 480 nm wavelength.35<\/a><\/sup><\/p>\n

MRI of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in Solution<\/h3>\n

The Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were mixed in 1 mL normal saline (NS) to obtain different Mn2\u00fe concentrations (0.12, 0.24, 0.59, 1.19, 1.78, and 2.38 mM), and pure NS was used as a control. All groups underwent 3.0 T MRI scanning. T2 images and T2 relaxation times with the following parameters were acquired using fast spin echo (FSE) sequences: repetition time (TR): 10, 20, 40, 60, 80, and 100 ms; echo time (TE): 10 ms. Different Fe3+<\/sup> relaxation times with different concentrations (0, 0.1, 0.2, 0.3, 0.4, and 0.5 mM) were obtained by linear fitting. The goodness-of-fit was calculated.<\/p>\n

Cytotoxicity of Fe3<\/sub>O4<\/sub>@CNSs and the Combination Chemo-PTT Therapy of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in vitro<\/h3>\n

The cytotoxicity of Fe3<\/sub>O4<\/sub>@CNSs was assessed using the Cell Counting Kit 8 (CCK-8) method. MCF-10A and 4T1 cells were seeded in 96-well plates (8000 cells per well) and treated with Fe3<\/sub>O4<\/sub>@CNSs in various concentrations (0, 50, 100, and 200 \u00b5g\/mL) for different durations (12, 24, 36, and 48 h); five compound wells were set up in each group. Subsequently, the wells were washed thrice with PBS, 100 \u00b5L CCK-8 was added, and the wells were incubated at 37\u00b0C for 40 min. The OD value was measured by a multifunctional microplate reader at 490 nm. The viability of the blank control group was considered as 100%.<\/p>\n

4T1 cells were seeded in 96-well plates (8000 cells per well) and treated with the culture medium containing the desired amount of free DOX (1.56, 3.12, 6.25, 12.5, and 25 \u00b5g\/mL) and Fe3<\/sub>O4<\/sub>@CNS (0, 50, 100, and 200 \u00b5g\/mL). For the photothermal heating experiments, after 4 h of incubation, excess unbound Fe3<\/sub>O4<\/sub>@CNSs or Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> was removed by rinsing three times with PBS. A fresh culture medium was then added into the wells. The cells were exposed to NIR light (808 nm laser, 1.5 W\/cm2<\/sup>) for 5 min to conduct photothermal or chemo-PTT treatments. Finally, the viabilities of the 4T1 cells were measured by a multifunctional microplate reader at 490 nm.<\/p>\n

Hemolysis Testing and in vitro Quantitative Cellular Uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

To test the hemolysis of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, 10 mL of rabbit blood was collected from the cardiac apex, following which 0.1 mL of potassium oxalate anticoagulant was added, and 10 mL NS was added to obtain diluted anticoagulant rabbit blood. Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were mixed into 1 mL NS to produce different concentrations (10, 50, 100, and 200 mg\/mL). NS was used as a negative control and double-distilled water (DDW) was used as a positive control. All tubes were placed in a 37\u00b0C water bath. After preheating for 30 min, 0.3 mL of anticoagulant rabbit blood was added to each tube and the solution was placed in a 37\u00b0C water bath for another 60 min. The samples were then centrifuged (1200 rpm, 5 min). The supernatant was transferred into a colorimetric dish to measure the OD at 545 nm. The hemolysis rate (HR) was calculated as HR (%) = (OD mean of the sample to be tested – OD mean of negative control)\/(OD mean of positive control – OD mean of negative control) \u00d7 100%. According to the GB\/T 14233.2 standard (Test methods for infusion, transfusion, injection equipment for medical use \u2013 Part 2: Biological test methods), if the HR is less than 5%, it indicates that the material has no hemolysis and meets the hemolysis test requirements of medical materials.<\/p>\n

To quantify the capacity of 4T1, MCF-10A, and THP-1 cells to intake Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, the three kinds of cells were inoculated into 6-well plates (3 compound wells were set up in each group). The medium was replaced with Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (80 mg\/mL), the control group used PBS, and the cells were incubated for another 4 h. Finally, the cells were washed thrice with PBS, digested with trypsin, centrifuged, counted, and dissolved in aqua regia for digestion for 3 days. The volume was constant at 5 mL, and the Fe content was analyzed by ICP-MS.<\/p>\n

Vitro and in vivo Photothermal Performance of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

Different concentrations of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (0, 100, 200, 400, and 600 \u00b5g\/mL) were irradiated by an 808 nm laser (1.5 W\/cm2<\/sup>) for 10 min in a quartz cuvette. During irradiation, the temperature of solutions was recorded using an IR thermal camera. Furthermore, to determine the photothermal stability, 100 mg\/mL of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> was irradiated for 5 min (808 nm laser, 1.5 W\/cm2<\/sup>) and naturally cooled. Ten cycles of control on\/off irradiation were performed with the laser (808 nm laser, 1.5 W\/cm2<\/sup>).<\/p>\n

All animal experiments were performed in compliance with the ethical guidelines established by the Ethics Committee on Scientific Research of Shandong University Qilu Hospital. Animal care was following the Laboratory Animals-Guidelines for ethical review of animal welfare in China (GB\/T 35892-2018). Female BALB\/c mice aged 6\u20138 weeks were purchased from Jinan Peng Yue Laboratory Animal Co., Ltd (Jinan, China). NS and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg) were injected into the tail vein of 4T1 tumor-bearing mice (4T1 cells were injected into the right hind legs of healthy BALB\/c mice for approximately 10 days). The tumors were irradiated by an 808 nm laser (1.5 W\/cm2<\/sup>) for 10 min, 24 h after injection. Thereafter, they were irradiated every 2 days and over a 14-day period. The tumor temperatures in the different groups were measured.<\/p>\n

Vivo MRI Monitoring<\/h3>\n

The in vivo monitoring experiments were performed using a 3.0 T MRI scanner on 4T1 tumor-bearing BABL\/c mice after tail vein injection with 0.1 mL Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg of body weight). The T2 sequence of the FSE sequence was TR: 2800 ms, TE: 72 ms; flip angle: 90\u00b0; slice thickness: 2.0 mm; and field of view: 120 mm. The MR images of mice were obtained at preinjection and different time intervals (0, 3, 12, 24, 48, and 72 h) after the injection of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/p>\n

Vivo Blood Circulation and Biodistribution of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

Biodistribution assays were performed on 4T1 tumor-bearing mice after tail vein injection of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg of body weight). Major organs (hearts, livers, spleens, lungs, kidneys, brains, and tumors) were harvested dried and ground at different time intervals (6, 12, and 24 h; 3 mice per interval) and dissolved by aqua regia for ICP-MS measurement to detect. Three 4T1 tumor-bearing mice injected with NS were used as the control. Additionally, blood was then collected 30 \u00b5L from the tail of the mice after injection of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg of body weight) at different time intervals (5 min, 15 min, 1, 2, 4, 8, 12, 24, and 48 h; 3 mice per interval). The Fe3+<\/sup> levels were measured by ICP-MS after blood samples were digested in aqua regia for 3 days. Finally, the amount of Fe3+<\/sup> in the above organs of interest is expressed in terms of the percentage if injection dose per gram (% ID\/g).<\/p>\n

In vivo Pharmacokinetics Study<\/h3>\n

All animal experiments were performed in compliance with the ethical guidelines established by the Ethics Committee on Scientific Research of Shandong University Qilu Hospital. Animal care was following the Laboratory Animals-Guidelines for ethical review of animal welfare in China (GB\/T 35892-2018). Healthy SD rats (200 \u00b1 20 g) were purchased from Jinan Peng Yue Laboratory Animal Co., Ltd (Jinan, China). Six male SD rats were randomly divided into two groups (n = 3), followed by tail-vein injection of free DOX and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> with doses of 3mg\/kg. Blood sample (0.3 mL) was taken from the eye socket of the SD rats at different times (0.17, 0.25, 0.5, 1, 2, 4, 8, 24 h) followed by a high-speed cryogenic centrifugation (15,000 rpm, 10 min). Plasma proteins were removed by methanol precipitation, and the concentrations of DOX were gauged by HPLC.<\/p>\n

In vitro and vivo Antitumor Efficiency of Chemo-PTT<\/h3>\n

A chemo-PTT experiment was performed by using double Hoechst\/propidium iodide (PI) staining. The 4T1 cells were seeded into 24-well plates, where a cell slide was placed to let the cells adhere. The cells were divided into 5 groups: NS with control group, DOX group, NIR group, Fe3<\/sub>O4<\/sub>@CNSs + NIR group, and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR group (4 mg Fe\/kg, DOX 200 \u00b5g\/mL). The cells in groups NIR, Fe3<\/sub>O4<\/sub>@CNSs + NIR, and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR were irradiated with an 808 nm laser (1.5 W\/cm2<\/sup>) for 10 min and incubated in a constant-temperature incubator for 1 h. Hoechst 33342 and PI dyes were added successively in the 24-well plates. In dark conditions, the cell slides were picked out with the needle tip of a syringe, and the cells with apoptosis and death were photographed using a biological microscope.<\/p>\n

The tumor sizes were measured and calculated as volume = (tumor length) \u00d7 (tumor width)2<\/sup>\/2. When the tumor volume of 4T1 tumor-bearing mice increased to approximately 150\u2013200 mm3<\/sup>, the mice were randomly divided into 5 groups (5 tumor-bearing mice per group): the grouping criteria were as above. Twenty-four hours after the tail vein injection of Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, mice from groups NIR group, Fe3<\/sub>O4<\/sub>@CNSs + NIR group, and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR were irradiated with an 808 nm laser (1.5 W\/cm2<\/sup>) for 10 min, once every 2 days, over a total of 14 days. The tumors were measured every 2 days over the 14-day period, and the changes in the tumor volume were observed. The mice in each group were euthanized according to standard animal protocols every 2 days, following which the tumors were removed and further hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were performed for histological examination. During the above different treatment methods, the body weight of mice was recorded every 2 days.<\/p>\n

Statistical Analysis<\/h3>\n

All the data were analyzed by SPSS 19.0 statistical software (SPSS, Inc., Chicago, IL, USA). Independent-sample t<\/em>-test, paired-sample t<\/em>-test, and analysis of variance were used to compare the differences between study groups. P<\/em> value <0.05 was considered statistically significant.<\/p>\n

Results and Discussion<\/h2>\n

Preparation and Characterization<\/h3>\n

Tumor-associated multifunctional theragnostic nanoplatforms have been in high demand in recent years for effective cancer therapy.35<\/a><\/sup> Herein, a low-toxicity multifunctional nanosystem, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, integrating chemo-PTT antitumor therapy and MRI monitoring, was successfully fabricated, and developed. In this synergistic anticancer therapy nanosystem, as illustrated in Figure 1<\/a>, every part plays its own roles: CNSs guarantees excellent Fe3<\/sub>O4<\/sub> and DOX loading, the CNSs and Fe3<\/sub>O4<\/sub> provide NIR photothermal conversion efficiency, the decorated Fe3<\/sub>O4<\/sub> facilitate Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> NPs for T2 enhanced MR imaging. In our previous study, we utilized CNS derived from glucose as the tumor cell-targeting template to load a low dose of manganese oxide and first report of a saccharide-based magnetic NP with long-circulation, self-sacrificial and tumor-targeting capabilities. CNS possesses the intrinsic biological characteristics of saccharide derivatives, including high biosafety, reticuloendothelial system (RES) escape and tumor targeting, long-circulation, intracellular self-degradation, and excellent stability.36<\/a><\/sup> Therefore, in our study, we utilized a CNS derived from glucose as the tumor cell-targeting template to load the DOX and Fe3<\/sub>O4<\/sub>.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 1<\/strong> Schematic illustration of biocompatible and double-triggering Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> nanosystem for combined chemo-PTT synergistic therapy and MR imaging monitoring.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The surface morphology features of the CNSs (Figure S1<\/a><\/u>) and Fe3<\/sub>O4<\/sub>@CNSs (Figure 2a<\/a>) were observed by TEM. The CNSs were capped with foreign balls, which suggested that Fe3<\/sub>O4<\/sub> was successfully loaded on the surface of the CNSs. The TEM, SEM and AFM of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were also obtained in Figure S2<\/a><\/u>, and the smaller particles represent the Fe3<\/sub>O4<\/sub>. By comparing with the TEM of Fe3<\/sub>O4<\/sub>@CNSs, the TEM of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> is almost unchanged. This is mainly because DOX is a molecule, which is difficult to cause the morphology change of nanomaterials. The XRD patterns of the prepared CNSs and Fe3<\/sub>O4<\/sub>@CNSs are shown in Figure 2b<\/a>. The diffraction peaks of the CNSs and Fe3<\/sub>O4<\/sub>@CNSs are located at ~22\u00b0, indicating that the caramelized nanospheres are in an amorphous state.24<\/a>,37<\/a><\/sup> The XRD peaks of the Fe3<\/sub>O4<\/sub>@CNSs can be indexed to the cubic phase [space group: Fd-3m (227)] of Fe3<\/sub>O4<\/sub> (JCPDS card no. 19-0629), suggesting that Fe3<\/sub>O4<\/sub> NPs are loaded on the surface of the caramelized nanospheres. The magnetic hysteresis loops of Fe3<\/sub>O4<\/sub> and Fe3<\/sub>O4<\/sub>@CNSs are shown in Figure 2c<\/a>. The saturation magnetizations are 62.5 emu\/g, and 44.6 emu\/g, respectively, indicating superparamagnetic. The Fe3<\/sub>O4<\/sub>@CNSs have a lower saturation magnetization than Fe3<\/sub>O4<\/sub>, which could be explained by the coating of Fe3<\/sub>O4<\/sub> NPs on the CNSs. The loading of Fe3<\/sub>O4<\/sub> on CNSs is determined to be 606.6 mg\/g.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 2<\/strong> (a<\/strong>) TEM images of Fe3<\/sub>O4<\/sub>@CNSs. (b<\/strong>) XRD patterns of the CNSs and Fe3<\/sub>O4<\/sub>@CNSs. (c<\/strong>) Magnetic hysteresis loops and saturation magnetizations of Fe3<\/sub>O4<\/sub> and Fe3<\/sub>O4<\/sub>@CNSs.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Drug Loading and Release Capacity of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

The surface zeta potential of the Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> changed from \u221229.2 mV to \u221227.5 mV after DOX conjugation (Figure 3a<\/a>). Fourier transform infrared (FTIR) spectroscopy was used to identify the structure and functional groups of the CNSs, Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (Figure 3b<\/a>). The peak of the FTIR spectra at 580 cm-1 is attributed to the Fe\u2013O stretching vibration. The peaks of CNSs, Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> at ~1704 cm-1 and 1625 cm-1 are assigned to C=O and C=C groups, respectively, which are formed by the caramelization of glucose.38<\/a><\/sup> The bands at ~3500 cm\u22121<\/sup> indicate \u2013OH stretching, and those in the 1000\u20131300 cm\u22121<\/sup> range for \u2013OH bending vibrations indicate the amount of \u2013OH on the surface of the CNSs and Fe3<\/sub>O4<\/sub>@CNSs, which would improve the hydrophilicity of the NPs.24<\/a><\/sup> Furthermore, compared to CNSs and Fe3<\/sub>O4<\/sub>@CNSs the FTIR spectra of the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (Figure 3b<\/a>) show a mew peak at 877 cm\u22121<\/sup>, which is assigned to \u2013NH2<\/sub>. In order to verify the connection mode of DOX with Fe3<\/sub>O4<\/sub>@CNSs, XPS analysis was carried out and no amide bond was found39<\/a><\/sup> from the Figure S3<\/a><\/u>. In addition, there was no peak located at around 1230 cm\u22121<\/sup> from IR spectrum of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in Figure 3b<\/a>, suggesting no C=ONH was formed.40<\/a><\/sup> The loading of DOX on [email protected]<\/a> may be mainly dependent on adsorption or incorporation because of the surface of Fe3<\/sub>O4<\/sub>@CNSs is negative charged while DOX is positive charged. Compared to Fe3<\/sub>O4<\/sub>@CNSs, the surface potential of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> decreased to \u221228.0 mV due to the positive charge DOX. Additionally, the zeta potentials of the [email protected]<\/a> and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were higher than 25 mV. According to DLVO theory, the zeta potential beyond \u00b1 25 mV is stable because of their electrostatic repulsion, thus preventing agglomeration.47<\/a><\/sup> However, the negative charge carriers are beneficial in transdermal and lymphatic drug delivery but reduced cellular uptake compared to positive charge carriers.41<\/a><\/sup> The surface zeta potentials and FTIR spectra indicate that the Fe3<\/sub>O4<\/sub>@CNSs are successfully loaded with DOX.42<\/a><\/sup> The weight of DOX in the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> was 29.8 mg\/g. Additionally, the zeta potentials of the Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were higher than 20 mV.\n<\/p>\n

The average hydrodynamic diameters of the CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were determined by dynamic light scattering (DLS) (Figure 3c<\/a>). This confirmed that the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> had the same dispersibility in colloidal solutions, as well as cell culture medium and had very little aggregation, rendering the method suitable for drug delivery applications.43<\/a><\/sup> The results can be ascribed to the surface charge of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> The average sizes of the CNSs, Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> were in the range of 150 nm\u2013180 nm (Figure 3c<\/a>) with the polydispersity index (PDI) of 0.156, 0.354 and 0.358, respectively. The vascular endothelial cell gap at the tumor site can be around 100\u2013780 nm, so nano-sized particles can enter the tissue through the vascular gap at the lesion, and when the NPs are loaded with drugs, it can achieve the greatest degree of blood circulation in solid tumors. Drug enrichment to achieve good therapeutic effect. NPs with a diameter in the range of 100\u2013200 nm can better pass through the tumor enhanced permeability and retention (EPR) effect and escape the filtration of the liver and spleen.44<\/a>,45<\/a><\/sup> Our Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> has an average diameter of 150\u2013180 nm, so it can better pass through the tumor EPR effect and effectively enrichment by tumor cells.<\/p>\n

To evaluate the loading capacity of DOX in the nanocarrier, we carried out drug release detection experiments. The in vitro DOX release profiles of the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> are pH\/NIR-dependent (Figure 3d<\/a>). Less than 10% of DOX is released at pH = 7.4 over 24 h, indicating relative stability under physiological conditions. The total drug release amount of the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in PBS solution (pH = 5) is 50.9%. Rapid drug release is observed under the 808 nm laser in the PBS solution (pH = 5), and a total drug release amount of 70.3% is obtained. This pH-responsive drug release is mainly attributed to two aspects: 1) Fe3<\/sub>O4<\/sub> could fall off the surface of CNS and dissolve at pH = 5, so DOX adsorbed to Fe3<\/sub>O4<\/sub> could release gradually; 2) The hydrophilicity and higher solubility of DOX increased in low-pH environments, which is caused by the increased number of protonated \u2013NH2<\/sub> groups in DOX.46<\/a><\/sup> At the same time, the TEM of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> after it was placed in PBS solution (pH = 5) for 24 h, demonstrating Fe3<\/sub>O4<\/sub> could dissolve (Figure S4<\/a><\/u>). For in vivo bio applications, this pH-responsive DOX release could ensure that the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> nanocomposites remain stable in the physiological environment (pH = 7.4). Additionally, the enhanced drug release upon NIR irradiation can be attributed to the increasing temperature generated by Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, which could directly increase the speed of desorption or release of DOX molecules for effective anticancer chemotherapy.47<\/a><\/sup><\/p>\n

Cytotoxicity of Fe3<\/sub>O4<\/sub>@CNSs and the in vitro Combined Chemo-PTT Therapy<\/h3>\n

Some studies have shown that nanomaterials such as those based on cashmere and copper sulfide can convert light into heat energy, leading to thermal damage, and are also quite toxic.48<\/a>,49<\/a><\/sup> According to our previous research, CNSs and Mn-loaded CNSs show low toxicity, and are a highly biocompatible nanomaterial for diagnostic imaging and therapy.24<\/a>,43<\/a>,50<\/a><\/sup> However, the neurotoxicity of Mn limits its clinical translation.24<\/a><\/sup> Fe3<\/sub>O4<\/sub>, as a clinically safe nanomaterial, has good biocompatibility.36<\/a>,51<\/a><\/sup> Therefore, we loaded Fe3<\/sub>O4<\/sub> and DOX on CNSs and determined the cytotoxicity to investigate the safety of Fe3<\/sub>O4<\/sub>@CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> MCF-10A and 4T1 cells were cultured with different concentrations of Fe3<\/sub>O4<\/sub>@CNSs (0, 50, 100, 150, and 200 \u00b5g\/mL) for different incubation times (12, 24, 36, and 48 h) and the standard cell survival rates were measured by CCK-8. The MCF-10A cells showed high cell survival rates. Even when the MCF-10A and 4T1 cells were cultured with Fe3<\/sub>O4<\/sub>@CNSs for 48 h at a concentration of 200 \u00b5g\/mL, the cell vitality was more than 85% (Figure 4a<\/a> and b<\/a>).\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 4<\/strong> MCF-10A (a<\/strong>) and 4T1 (b<\/strong>) cell lines incubated with Fe3<\/sub>O4<\/sub>@CNSs at different concentrations (0, 50, 100, 150, and 200 \u00b5g\/mL) for various durations (12, 24, 36, and 48 h). (c<\/strong>) In vitro cytotoxicity of Fe3<\/sub>O4<\/sub>@CNSs, Fe3<\/sub>O4<\/sub>@CNSs + NIR, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, pure DOX, and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR against 4T1 cells after 24 h of incubation.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The cytotoxic effect of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> on 4T1 cells under NIR light irradiation was investigated by CCK-8 tests. In vitro cytotoxicity of each group after treatment with Fe3<\/sub>O4<\/sub>@CNSs, Fe3<\/sub>O4<\/sub>@CNSs+NIR, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a>, pure DOX and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR for 24 h respectively, the evaluation was carried out and the results are shown in Figure 4c<\/a>. The cells treated with Fe3<\/sub>O4<\/sub>@CNSs alone exhibited no significant cell death for all concentrations. The cells incubated with Fe3<\/sub>O4<\/sub>@CNSs + NIR exhibited moderate cell death, with almost 63% of the cells still viable up to a concentration of 200 \u03bcg\/mL. The Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> had comparable cytotoxicity compared with pure DOX, indicating that the DOX-loaded CNS was pharmacologically active as a potential drug carrier. Cells cultured with Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> and exposed to laser irradiation for 10 minutes had a much higher number of deaths than any of the above, with less than 30% surviving cells at 200 \u03bcg\/mL. The increased cell ablation could contribute to better therapeutic effects in combined chemo-PTT.<\/p>\n

Hemolysis Rates, Quantitative Cellular Uptake and MRI Monitoring Capability of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

The absorbance value of each group at 545 nm was used to calculate the HR of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> at different concentrations (10, 50, 100, 200 \u00b5g\/mL) which were ranged from 0.79% to 1.95% (Table S1<\/a><\/u>), far less than 5.0%. There was almost no hemolysis in the nanomaterials of different concentrations (Figure S5<\/a><\/u>). Thus, the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> had no hemolysis effect and conforms to the hemolysis requirement for medical materials.<\/p>\n

The pre- and post-intake Fe contents were 4.60 \u00b1 0.08 and 4.87 \u00b1 0.19 pg for MCF-10A; 2.67 \u00b1 0.55 and 4.89 \u00b1 1.41 pg for macrophages; 4.22 \u00b1 0.14 and 8.70 \u00b1 0.91 pg for 4T1, respectively. No difference was observed between the pre- and post-uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in MCF-10A cells (p<\/em>=0.08). However, differences were noted between the pre- and post-uptake in 4T1 (p<\/em><0.01) and macrophages (p<\/em>=0.04) (Figure 5a<\/a>). This indicates that MCF-10A had limited uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> compared with human macrophages and 4T1 cells. The possible reason was that CNSs were obtained through caramelization reaction of anhydrous glucose.52<\/a><\/sup> The expression of glucose transporter (Glut1) receptor is increased in breast cancer. We speculated that the uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> is related to the expression of more Glut1 on the surface of tumor cell membranes to meet its growing energy demands.53<\/a>,54<\/a><\/sup> Compared with tumor cells, there are fewer glucose transporter receptors on the surface of MCF-10A. Therefore, there is less uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> than 4T1. Moreover, the average size of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> is 150 nm\u2013180 nm, while particles of 200 nm or greater in size are more efficiently taken up by the reticuloendothelial system (RES) rich in macrophages.55\u201357<\/a><\/sup> So Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> could partly avoid amounts of uptake by RES.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 5<\/strong> (a<\/strong>) Cellular pre- and post-uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> by MCF-10A, macrophages and 4T1 cells. (b<\/strong>) Linear relationship of r2<\/sup> (1\/T2) values vs concentration of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> mainly affected the relaxation time of the T2 image, which had a small effect on the T1 image and could achieve a negative enhancement effect. As shown in Figure 5b<\/a>, the T2 images become dark gradually as the concentration of the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> increase. Additionally, in comparison to the previous reported,35<\/a><\/sup> the r value of the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> can reach 15.291 (mg\/mL)\u22121<\/sup>S\u22121<\/sup>, and we have similar R2<\/sup> values. This indicates that the synthesized material has a better ability to shorten T2. This might be due to that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> could provide the advantages of proper size and shape, which can increase the chance of improving the rate of relaxation rates and the ideal modification of the contrast agent label.58<\/a><\/sup><\/p>\n

Vitro and vivo Photothermal Effect of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

Typically, cancer cells are more sensitive to heat than normal cells due to the immature vasculature structure. Many studies have reported that cancer cells can be killed by being kept at 42\u00b0C for 15\u201360 min or only 4\u20136 min when the temperature exceeds 50\u00b0C.59<\/a>,60<\/a><\/sup> However, high temperatures (52\u00b0C) cause irreversible damage to normal cells.61<\/a><\/sup> Thus, mild PTT is a promising cancer treatment for the minimal damage to the normal cells.<\/p>\n

The Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> aqueous solution has a wide absorption range (400\u20131000 nm) from visible light to near-infrared light (Figure S6<\/a><\/u>). This shows that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> have the theoretical basis for near-infrared photothermal conversion.38<\/a><\/sup> The photothermal conversion of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in aqueous solution was achieved by irradiation with NIR (808 nm, 1.5 W\/cm2<\/sup>). With the extension of irradiation time (0, 120, 240, 360, 480 s) and the increase in Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> solution concentration (100, 200, 400, 600 \u00b5g\/mL), the temperature of the Fe3<\/sub>O4<\/sub>@CNSs solution increased significantly (Figure 6a<\/a>), and corresponding temperature curve is shown in Figure S7<\/a><\/u>. Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (0 and 100 \u00b5g\/mL) solutions show minimal temperature change (rising to 37\u00b0C and 41\u00b0C, respectively) after 5 min of irradiation. The temperature of 200 \u03bcg\/mL Fe3<\/sub>O4<\/sub>@CNSs increased to 45\u00b0C, which is sufficient to destroy tumor cells.8<\/a><\/sup> The 400 \u00b5g\/mL and 600 \u00b5g\/mL Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> solution temperatures reached 50\u00b0C and 56\u00b0C, respectively, after 5 min of irradiation. To further analyze the photothermal stability of Fe3<\/sub>O4<\/sub>@CNSs, a near-infrared laser (808 nm, 1.5 W\/cm2<\/sup>) irradiated 100 \u00b5g\/mL Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> for 5 min, which was then cooled to room temperature and then further irradiated for 5 min for a total of ten cycles (ie, 100 min irradiation). The results showed that the photothermal conversion ability of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> did not decrease after ten cycles (Figure 6b<\/a>). This indicates that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> have good photothermal conversion efficiency and stability and show good potential as a photothermal agent.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 6<\/strong> (a<\/strong>) Infrared thermal images of NS and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (b<\/strong>) The temperature change of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> solution (100 mg\/mL) after turning cycling the laser four times. (c<\/strong>) Thermographs of the tumor region after intravenous injection with NS and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (d<\/strong>) Heating curve of the various groups.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The photothermal effect of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> was excellent in vitro, and we further explored the feasibility of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> as a photothermal agent to treat tumors in vivo. Tumor-bearing mice were injected into the tail vein with CNSs and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg, 3 mice per group). An 808 nm laser (1.5 W\/cm2<\/sup>) was used for 10 min of irradiation, and the temperature change was observed (Figure 6c<\/a> and d<\/a>). In the NS group, the temperature in the tumor areas was raised to 41.5\u00b0C, however, a rapid temperature increase from 29.3\u00b0C to 60.8\u00b0C was observed for tumors in the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> group.62<\/a><\/sup> Under the same irradiation conditions, the tumor temperature of mice injected with NS was significantly lower than that of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> This shows that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> have a relatively efficient thermal effect and could be used as a PTT agent for in vivo tumor ablation.<\/p>\n

In vivo MRI Imaging of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

In recent years, many researchers have focused on assisted NIR nanocarriers with imaging monitoring for precise cancer diagnosis and location in order to guide external laser irradiation without damaging the surrounding healthy tissue.63<\/a><\/sup> We further studied the in vivo MRI performance of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> The in vivo MR imaging was performed on 4T1 tumor-bearing BABL\/c mice after intravenous injection of 0.1 mL Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg) and scanned with a 3.0 T MRI scanner. Figure 7<\/a> shows MR images of mouse tumors (red circles) at different time intervals. The T2 signal intensity changed in the tumor after injection of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (Figure S8<\/a><\/u>). Within 24 h after Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> injection, the T2 signal intensity in the tumor gradually decreased. During this period, the overall background signal intensity of the mice also gradually decreased, and after 24 h, the T2 signal intensity of tumor gradually increased. Thus, the Fe3<\/sub>O4<\/sub>@CNSs could help to integrate both MRI monitoring and chemo-PTT into a single multimodal nanosystem to construct a theragnostic platform, which can help to diagnose and locate the cancer and to visualize NP accumulation.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 7<\/strong> In vivo T2 MRI of 4T1 tumor-bearing mice at different time intervals (0, 3, 12, 24, 48 and 72 h) after injection with Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> (4 mg Fe\/kg).<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

In vivo Blood Circulation of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> and Clearance of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a><\/h3>\n

In Figure 8a<\/a>, the blood concentration of Fe3+<\/sup> decreases gradually and is retained for more than 24 h, it was confirmed that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> has a long blood circulation, which can delay the clearance of NPs by macrophages in the reticuloendothelial system64<\/a><\/sup> and is more beneficial to the large uptake of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> by tumor tissue through EPR effect. This is consistent with the lowest T2 signal intensity of the tumor tissue 24 h after the tail vein injection of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in 4T1 tumor-bearing mice. Figure 8b<\/a> shows that Fe3+<\/sup> accumulates in most organs (heart, liver, spleen, kidney, and lungs), while the tumor exhibits the most Fe3+<\/sup> uptake. The tumor uptake of Fe3+<\/sup> which can be associated with the EPR effect. In addition, as mentioned above, 4T1 cells that express a large amount of Glut on the surface contribute to the uptake large amounts of glucose. We can also find kidney tissues accumulated much more Fe3+<\/sup>. The kidney uptake of Fe3+<\/sup> can be contributed to possible renal excretion.65<\/a><\/sup>\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 8<\/strong> (a<\/strong>) Blood circulation and (b<\/strong>) biodistribution of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> observed after intravenous injection in mice. Fe concentration measurements were performed by ICP- MS. (c<\/strong>) Pharmacokinetic of free DOX and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> after a single dosage intravenous to rat.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

In vivo Pharmacokinetics Study<\/h3>\n

The pharmacokinetics behavior of DOX was studied in rats through the tail-vein injection of free DOX and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> The concentration of DOX in plasma at different times is shown in Figure 8c<\/a>. Compared with free DOX, the pharmacokinetic characteristics of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> in vivo were significantly changed based on the pharmacokinetic parameters of the two groups as presented in Table 1<\/a>. At the same dose, the elimination half-life time (t1\/2\u03b2) of DOX in Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> group was significantly increased by 11.18h compared with free DOX group. The clearance rate (CL) of free DOX group was 2.61 times as much as Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> group, respectively. The decreases of in vivo CL in Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> group might be due to the increased blood stability by escaping the RES and the sustained release of DOX from NPs. In addition, the mean retention time (MRT), area under the curve AUC0<\/sub>\u2192t<\/sub> and AUC0<\/sub>\u2192\u221e<\/sub> values of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> group were significantly increased by 0.51, 1.31 and 1.65 -fold, respectively, compared with free DOX group. These results demonstrated that Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> with a sustained release property could significantly improve the bioavailability of DOX.66<\/a><\/sup>\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Table 1<\/strong> Pharmacokinetic Parameters of DOX and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> After a Single Dosage Intravenous to Rat<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Vitro and vivo Therapeutic Efficacy of Chemo-Photothermal Treatment<\/h3>\n

Segy et al67<\/a><\/sup> and many others have shown that combining Fe3<\/sub>O4<\/sub> with PTT allows diagnosis and treatment; however, the studies of these researchers did not combine photothermal treatment with chemotherapy to achieve a combined therapeutic effect.32<\/a>,68<\/a><\/sup> Here, we combined the two treatments and verified their effect. Since CNSs and Fe3<\/sub>O4<\/sub> have been proven to have photothermal effects, no separate group was required.43<\/a>,51<\/a><\/sup><\/p>\n

To confirm the efficacy of Fe3<\/sub>O4<\/sub>@CNSs PTT combined with DOX in the treatment of tumors, 4T1 cells (including living, apoptotic, and dead cells) were stained with Hoechst 33342 and PI (Figure 9a<\/a>). The survival rate of the control group was up to 99%. The DOX group showed a few dead cells. The NIR and Fe3<\/sub>O4<\/sub>@CNSs + NIR groups showed partial dead cells, but Fe3<\/sub>O4<\/sub>@CNSs + NIR showed greater potential for killing cells. Hence, Fe3<\/sub>O4<\/sub>@CNSs have photothermal superposition ability, rendering PTT an effective strategy for tumors. When tumor cells were exposed to DOX combined with Fe3<\/sub>O4<\/sub>@CNSs and subjected to laser irradiation almost all cells died, indicating that DOX combined with PTT could effectively induce cell death and achieve effective treatment.\n<\/p>\n\n\n\n
\"\"<\/a><\/td>\n\n

Figure 9<\/strong> Hoechst 33342\/PI fluorescence staining of 4T1 cells after different treatments with NS as control, DOX, NIR, Fe3<\/sub>O4<\/sub>@CNSs + NIR, and Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR, respectively (a<\/strong>), scale bar = 100 \u00b5m. The relative tumor volume (b<\/strong>) and body weights curves (c<\/strong>) of mice after different treatments (*p<\/em><0.05). H&E-stained images of tumors from different groups (d<\/strong>), scale bar = 100 \u00b5m.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Over 14 days, the tumor volume of the control group increased by 12.8 times, that of the DOX group increased by 10.4 times, and that of the NIR group increased by 7.5 times, indicating that, compared with chemotherapy, NIR has the highest potential to kill tumor cells. The traditional chemotherapy drug, DOX, also influenced the tumor volume. However, DOX has known toxicity. After 10 days of DOX injection, the mice were in poor health, and some even died. Fourteen days after Fe3<\/sub>O4<\/sub>@CNSs + NIR treatment, the tumor volume increased by 2.3 times; after 14 days of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR treatment, the tumor volume decreased by 32%. Thus, chemotherapy combined with PTT may effectively inhibit tumor growth and have better antitumor effects than either treatment alone (Figure 9b<\/a>). Additionally, all mice remained healthy, suggesting that the mice were neither starving nor diseased.69<\/a><\/sup> During the experiments, the body weights and tumor volumes of mice were monitored every two days and the results are recorded in Figure S9<\/a><\/u>. The variation trends of the mean body weights (Figure 9c<\/a>) show that the weights of mice in all seven groups increased with a similar increase trend. However, the weight gain of mice in Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR group was the slowest, which may be related to its effective therapeutic effect. The tumors of the different groups were analyzed histologically and stained with H&E and TUNEL. After staining, the nuclei of the control group were large, dark, and dense, without distinct damage. The DOX group had a few abnormal and dead cells. The Fe3<\/sub>O4<\/sub>@CNSs + NIR group exhibited necrosis, the tumor cells had lost their cell morphology, and there was evidence of lamellar necrosis. In the Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> + NIR group, massive necrosis was evident, and features related to necrosis could be clearly observed, such as cytoskeleton and nuclear disintegration (Figure 9d<\/a>, Figure S10<\/a><\/u>).<\/p>\n

Organ histology (Figure S11<\/a><\/u>) of the mice was performed to exclude the possibility of induced toxicity. Serious irreversible pathological alterations and injuries to the organs of mice in all groups were eliminated through H&E analysis, demonstrating the safety of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> during medicating photothermal and chemical ablations. Therefore, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> could act as a powerful chemo-PTT agent in vivo.<\/p>\n

Conclusion<\/h2>\n

In this work, the multifunctional nanosystem of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> could simultaneously achieve NIR-responsive chemo-PTT and MRI monitoring to enhance the therapeutic efficacy for TNBC. In vivo, Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> had longer circulation with higher AUC compared with DOX. The photothermal effect of Fe3<\/sub>O4<\/sub>\/[email protected]<\/a> under 808 nm irradiation can not only rapidly heat up tumor tissue and \u201ccook\u201d cancer cells, but also promote the release of DOX, thereby providing synergy for 4T1 cells in vitro. Pathological results showed that the heart, lung, kidney; liver, spleen and other organs had no obvious lesions. In summary, the system has the properties of easy synthesis, good biocompatibility, and low toxicity. The effective monitoring of MRI and the effect of combined chemo-PTT therapy will provide a new way for the treatment of TNBC.<\/p>\n<\/div>\n

\n

Acknowledgment<\/h2>\n

This research was supported by the National Natural Science Foundation of China (NSFC81771888).<\/p>\n

Disclosure<\/h2>\n

The authors declare no competing financial interest.<\/p>\n

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