{"id":55190,"date":"2024-12-25T10:09:34","date_gmt":"2024-12-25T11:09:34","guid":{"rendered":"https:\/\/peymantaeidi.net\/stem-cell\/?p=55190"},"modified":"2024-12-25T11:40:20","modified_gmt":"2024-12-25T11:40:20","slug":"color-changing-test-for-rapid-salmonella-detection","status":"publish","type":"post","link":"https:\/\/peymantaeidi.net\/stem-cell\/2024\/12\/25\/color-changing-test-for-rapid-salmonella-detection\/","title":{"rendered":"Color-Changing Test for Rapid Salmonella Detection"},"content":{"rendered":"
\n

A group of chemists at the University at Albany have developed a new method for fast-acting Salmonella<\/a> detection. The test employs a paper strip that changes color in the presence of the bacterial genome, enabling quick screening for salmonella in food products. <\/p>\n<\/p><\/div>\n

\n
\n
\n
\n

<\/i>\n <\/div>\n

\n

Yigit demonstrates an earlier iteration of his lab’s color-change technology wherein salmonella DNA triggered the test solution to turn purple.<\/p>\n

Patrick Dodson<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/p><\/div>\n<\/p><\/div>\n

\n

Unlike other methods, which can take days to yield a result, this system can detect salmonella under four hours. It is also able to identify and differentiate among two common salmonella strains, and costs less per test than current methods. The work was published in Advanced Healthcare Materials<\/em> <\/em>and was featured on the journal\u2019s September cover. <\/p>\n<\/p><\/div>\n

\n

\u201cSalmonella is one of the most common foodborne pathogens, making it a leading concern for food safety worldwide,\u201d said Mehmet Yigit, associate professor in UAlbany\u2019s Department of Chemistry and the RNA Institute, both part of the College of Arts & Sciences. \u201cContamination occurs when food comes into contact with animal feces during production, processing or handling. Products like raw or undercooked meat, poultry, eggs, seafood and fresh produce are common sources. Even processed items like peanut butter or frozen meals have been linked to outbreaks.<\/p>\n<\/p><\/div>\n

\n

\u201cIn this study, we demonstrate a new method for salmonella detection that uses a novel combination of molecular approaches to signal contamination in a food sample. Designed to be simple, fast and versatile, our method addresses a critical need for quicker, more accessible ways to identify foodborne pathogens. Being able to rapidly identify and isolate the source of a pathogen can reduce illness spread, minimize risks to vulnerable populations and reduce disruptions throughout the food supply chain.\u201d<\/p>\n<\/p><\/div>\n

\n

Salmonellosis, the illness caused by ingesting salmonella, can trigger acute gastrointestinal issues and fever. While the illness can resolve without treatment, severe infections can require hospitalization, especially in young children, the elderly or those with weakened immune systems. According to the U.S. Centers for Disease Control and Prevention, around 1.35 million cases of salmonellosis and 420 related deaths occur in this country every year.<\/p>\n<\/p><\/div>\n

\n

For food distributors and restaurants, monitoring for salmonella is both a health imperative and a business priority. Outbreaks are expensive; they can trigger costly product recalls, loss of consumer trust and even legal liabilities, disrupting operations with potential for long-term financial and reputational impacts. <\/p>\n<\/p><\/div>\n

\n

Honing Precise and Affordable Detection <\/h2>\n

The team\u2019s method uses CRISPR-Cas12a gene editing technology, combined with recombinase polymerase amplification (RPA) and a molecular tool called a \u201ctoehold switch\u201d to trigger a visible color change on a paper test strip. In the presence of salmonella, the strip turns red, signaling contamination. If it turns yellow, the sample is clean.<\/p>\n<\/p><\/div>\n

\n

This approach is not only quick and sensitive but also highly specific. The test can detect as few as 100 genome copies of salmonella bacteria and can distinguish between two common serotypes: S. Typhimurium<\/em> and S. Enteritidis<\/em>. Identifying the strain type can help track the origin of an outbreak and can dictate the optimal clinical approach to treat an infection. <\/p>\n<\/p><\/div>\n

\n

\u201cA strength of our system is its ability to minimize false-positive results,\u201d Yigit said. \u201cOther approaches involving nanoparticle-based or instrumental techniques often can\u2019t differentiate between pathogens, which reduces practical efficacy in the field.<\/p>\n<\/p><\/div>\n

\n

\u201cOur method also offers cost savings. By using tiny paper discs as the medium for detection, rather than a vial of reaction solution, a much smaller amount of test solution is required to achieve a result. This makes our method about 20 times less expensive per test.\u201d<\/p>\n<\/p><\/div>\n

\n

A Portable, Adaptable Test Kit <\/h2>\n

Unlike traditional methods, this test eliminates the need for extensive laboratory equipment or re-engineering for different bacterial strains, making it possible to implement in a variety of settings including farms, food processing plants, distribution facilities or restaurants. <\/p>\n<\/p><\/div>\n

\n

\u201cWith further development, we aim to transform this technology into a portable, user-friendly kit that can be used widely in the food supply chain,\u201d said lead author Mahera Kachwala, who worked on the project while completing her PhD with Yigit at UAlbany. \u201cAnyone handling food will be able to easily integrate this test into daily on-site operations, making it possible to stop a salmonella outbreak before it spreads. It could also become a useful tool for restaurant workers and health inspectors, to help ensure that routine hygienic protocols are being employed effectively, and customers are receiving safe, high-quality food.\u201d <\/p>\n<\/p><\/div>\n

\n

The system\u2019s design is also highly adaptable. By changing a single RNA element, it can be programmed to detect other pathogens responsible for food-borne illnesses. For example, the team is exploring ways to use their platform to test for additional types of salmonella, campylobacter and Shiga toxin-producing E. coli <\/em>(STEC), which together account for millions of hospitalizations and billions in lost food production costs each year. <\/p>\n<\/p><\/div>\n

\n

\u201cOur method demonstrates the power of synthetic biology to create flexible tools for pathogen detection,\u201d Yigit said. \u201cBy collaborating with experts in nanotechnology and microbiology, we are excited to bring this innovative technology closer to portable, consumer-ready use.\u201d<\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

A group of chemists at the University at Albany have developed a new method for<\/p>\n","protected":false},"author":1,"featured_media":55192,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/posts\/55190"}],"collection":[{"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/comments?post=55190"}],"version-history":[{"count":2,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/posts\/55190\/revisions"}],"predecessor-version":[{"id":55193,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/posts\/55190\/revisions\/55193"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/media\/55192"}],"wp:attachment":[{"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/media?parent=55190"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/categories?post=55190"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/peymantaeidi.net\/stem-cell\/wp-json\/wp\/v2\/tags?post=55190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}