Gold Nanoparticles Coated with SH-PEG-NH2 and Loaded with Ziyuglycoside I for Promoting Autophagy in Hematopoietic Stem Cells

Introduction

Myelosuppression is a severe adverse reaction caused by chemotherapy and radiotherapy that might affect patients receiving treatment for malignancies. A decrease in peripheral blood hemocyte counts, including leucocyte, erythrocyte, platelet, and neutrophil counts, is the classic indicator of myelosuppression. This frequently causes infection, bleeding, or anemia in cancer patients. To end myelosuppression, effective therapeutic options are urgently needed.^1,2

In recent years, autophagy has been shown to play an important role in the life course of haematopoietic stem cells(HSCS), which is closely associated to the properties of HSCS (self-renewal,³ pluripotency,⁴ differentiation,⁵ and resting state).⁶ Both Jacquel et al and Warr et al^7,8 showed that autophagy is also an HSCS survival mechanism under radiotherapy and chemotherapy harm. Additionally, cytoprotective autophagy can increase HSCS’s ionizing radiation tolerance. It is clear that autophagy is crucial for maintaining the HSCS’s life processes and activities. In order to increase hematopoietic stem cells’ resistance to chemoradiotherapy, it is crucial to increase autophagy in these cells.

Ziyuglycoside I (ZgI) is an active component extracted from the traditional Chinese medicinal Sanguisorba officinalis L, it increases leucocytes and protects hematopoietic stem cells.⁹ In a recent work, we found that ZgI can drastically downregulate the expression of mTOR protein in HSCs in myelosuppressed mice while significantly upregulating the expression of Atg5, Atg7, Beclin-1, LC3B, and other proteins in HSCS.¹⁰ What’s more, we simulated the molecular docking of ZgI and mTOR by computer. It was found that ZgI and mTOR could bind well, and multiple hydroxyl groups in ZgI structure interacted with LYS105, GLY101, LYS89 and ASN39 in mTOR kinase to generate multiple hydrogen bonds, as shown in Figure 1. This interaction increased the binding force between ZgI and mTOR protein. It has been hypothesized that ZgI may be an autophagy activator, and that its ability to increase leukocytes and defend HSCs may be connected to encouraging HSC autophagy. However, previous studies have shown that ZgI has poor water solubility and low oral bioavailability, which may adversely affect its efficacy.¹¹ Therefore, modern preparation methods are needed to improve the above problems in order to improve its efficacy and activity.

Figure 1 The chemical structure of Ziyuglycoside I its interaction with mTOR.

AuNPs (Gold nanoparticles, AuNPs), as a new type of drug delivery system, are widely used in the field of pharmacy due to their good biocompatibility, targeting, and easy surface modification.^12,13 In addition, studies have confirmed that AuNPs is an autophagy regulator that can promote autophagy.^14,15 Hence, it can be used as drug carriers to enhance autophagy. Therefore, we chose AuNPs as the carrier of ZgI in order to enhance the protective autophagy of HSCs.

This paper aims to covalently combine ZgI and gold nanoparticles to create ZgI-AuNPs, as is shown in Figure 2, and study its effect on autophagy in HSCS. This is based on the previous research that ZgI protects HSCS by regulating autophagy, combined with the excellent biological properties of AuNPs and the promotion of autophagy.

Figure 2 Preparation scheme of ZgI-AuNPs.

Materials and Cell Lines

Ziyuglycoside I was purchased from MUST Biotech Co, Ltd. (Chengdu, China). Sodium citrate Regent Chemicals Co., LTD (Tianjin, China). Tetrachloroauric acid (HAuCl₄) was purchased from Sigma company. The SH-PEG-NH₂ was purchased from Aladdin Chemical Reagents Co., LTD (Beijing, China). The FBS and DMEM high-sugar medium were purchased from Gibco (USA). The CCK-8 were purchased from Procell Life Science &Technology Co., Ltd (Wuhan, China). LC-3B and P62 antibody Kit was purchased from Abcam co.ltd (England). Cyclophosphamide(CTX) was kindly provided by Affiliated Hospital of Zunyi Medical University. Chloroquine(CLQ) was purchased from Sigma(USA).

Human hematopoietic stem cells (HSPC-1) cells were kindly provided by Procell Life Science &Technology Co., Ltd (Wuhan, China).

Preparation of ZgI-AuNPs

AuNPs were prepared by HAuCl₄-sodium citrate reduction method.¹⁶ The optimized ZgI-AuNPs formula, (HAuCl₄ (1% w/w), sodium citrate (38.8mM), SH-PEG-NH₂ (0.25 mM), ZgI amount (100μg·mL⁻¹), was the most convenient one with drug content of 15.63μg/mg. Briefly, 38.8mM sodium citrate was added into 1% aqueous solution of HAuCl₄, and the mixed solution was stirred on a magnetic stirrer for 6 hours to obtain gold nanoparticles(AuNPs), which was red colloid solution. Then 2.4 mL of 0.25 mM aqueous solution of HS-PEG-NH₂ was mixed with 6 mL of AuNPs colloid solution, and the mixed solution was supplemented with deionized water to 12 mL, stirring at room temperature for 30 min and putting it in a refrigerator at 4 °C overnight. Then the ZgI of 100μg·mL⁻¹ was added and incubated in a constant temperature water bath at 37°C for 12 hours. The mixed solution was centrifuged for 30 minutes at a speed of 10,000 rpm to collect precipitates and remove the unreacted free ZgI after incubation.

Characterization of ZgI-AuNPs

At room temperature (25°C), a laser particle analyzer (90Plus PALS, Brookhaven, USA) was used to assess the zeta potential and particle size of ZgI-AuNPs. Prior to the assays, samples were ten times diluted in phosphate buffer solution (pH=7.4). Transmission electron microscopy (TEM, JEM-2100Plus; JEOL, Japan) and scanning electron microscopy (SEM, S4800; HITACHI, Japan) were used to detect the morphology of ZgI-AuNPs.

Thermo Fisher Scientific, USA) used ultraviolet spectrometry to measure the drug loading (DL) and encapsulation efficiency (EE) of ZgI-AuNPs.¹⁷ In a nutshell, PBS was used to dissolve the ZgI-AuNPs powder, and the absorbance of ZgI was measured at 204 nm. The Thermo Fisher, USA’s Nicolet 6700 Fourier transform infrared spectroscopy was employed to ascertain ZgI’s physical condition inside the AuNPs. The pristine ZgI, physical mixture of ZgI and AuNPs and ZgI-AuNPs were detected and compared.

Radiation Diffraction (XRD). The structural features of the materials were determined using a D8 Focus X-ray diffractometer from Bruker in Germany that was equipped with Cu-K radiation. The voltage and current used for the experiments were 35 kV and 40 mA. From 5° to 90°, samples were scanned at a rate of 6°/min.

Analysis using a differential scanning calorimeter (DSC). A DSC 250 differential scanning calorimeter (TA Instruments, USA) was used for DSC. Before testing, samples were placed in an aluminum sample pan and dried at 60 °C for 1 hour. The analysis was conducted with a flow rate of 20 mL/min and a heating rate of 10 °C/min in a nitrogen environment. In the temperature range of −40 °C to 400 °C, the thermogram was recorded.

Cell Culture and Cell Viability Assay

In order to support the development of CD34⁺ hematopoietic stem cells, HSPC-1 cells were cultivated in RPMI-1640 with 10% fetal bovine serum along with 100 U/mL of penicillin and 100 g/mL of streptomycin. By using the CCK-8 assay, the cytotoxicity of ZgI-AuNPs was evaluated. By measuring absorbance at 450 nm in a microplate reader, the OD values were determined (MK3, Thermo, USA).

In vitro Release

The in vitro release of ZgI and ZgI-AuNPs was carried out on a thermostatic oscillator (Changzhou Enpei Instrument Manufacturing Co., LTD, China). The ZgI and ZgI-AuNPs were dispersed in water, shaking at a speed of 100 r· min⁻¹ and at a temperature of (37±0.5) °C. 1 mL release medium was taken out at 0.5, 2, 4, 6, 8, 10, 12, 24, 36 and 48 h. Then the release medium was centrifuged at 10,000 r min⁻¹ for 15 min and the absorbance of supernate was measured at 254 nm. At the same time, equal volume of isothermal release medium was replenished. The cumulative drug release rate (Q) at different time points was calculated according to following formula, and the cumulative release curve was drawn.

Where V₀ is the total volume of the release medium (mL), C_i and C_n are the drug concentration (mg·mL⁻¹) measured at the i-hr and n-hr time points, V_i is the sampling volume (mL), and m is the total mass of ZgI in the samples.

Effect of ZgI-AuNPs on the Autophagy of HSPS in vitro

Hematopoietic stem cells were injured in vitro using the chemotherapy drug cyclophosphamide (CTX). A 96-well plate containing HSPC-1 cells was seeded with 1×10⁵ cells per well. The settings for the experimental groups were the normal control group (NC, saline + RPMI 1640), CTX (CTX + RPMI 1640), ZgI and ZgI-AuNPs treatment group (CTX + RPMI 1640 supplemented with ZgI and ZgI-AuNPs of 5 μg·mL⁻¹ concentration, respectively). CTX was applied to cells in all other groups excluding the NC group at a concentration of 1 M. After 36 hours of incubation, the supernatant from each group was collected, and the appropriate drugs were then added. Cell viability was assessed after a 24-hour treatment using the CCK-8 test.

TEM was used to study the autophagy condition of HSPC-1 cells. The HSPC-1 cells were rinsed with PBS, fixed for 4 hours in 2.5% glutaraldehyde in PBS, and then post-fixed for another 2 hours in 1% OsO₄ in PBS. The samples were dried using ethanol gradients before being transferred to absolute acetone for 20 minutes. The samples were placed in a 1:1 mixture of absolute acetone and the final Spurr resin (SPI-CHEM, 02690-AB) mixture for one hour at room temperature, then moved to a 1:3 mixture of absolute acetone and the final resin mixture for three hours, and finally to the final Spurr resin mixture for an overnight period. Under transmission electron microscopy, the ultrastructures of the cells were studied.

By using immunofluorescence analysis, the protein LC3B that is connected to autophagy was detected. In a nutshell, HSPC-1 cells were washed in PBS, fixed for 20 min at room temperature with 4% paraformaldehyde, and then permeabilized for 10 min with Triton X-100 (Solarbio, T8200). Cells were first blocked for 1 hour with the immunostaining blocking buffer (Beyotime Biotechnology, P0252), and then treated with primary Anti-LC3B (Abcam, ab192890, 1:1000) antibodies overnight at 4°C. The secondary goat anti-rabbit antibodies (Solarbio, SE134; 1:1000) were incubated for 1 hour at room temperature. DAPI (Solarbio, C0065) was used to stain the nuclei for five minutes at room temperature. Finally, cells from each group were examined using a fluorescent microscope (CKX53IPC; Olympus, Japan), and the accompanying cellsens standard software was used to calculate the relative fluorescence intensity.

Effect of ZgI-AuNPs on the Autophagy of HSPs in vivo

SPF grade male and female KM mice (aged 5 weeks, weighing 20 ± 2 g) were purchased from Tianqing Biotechnology Co., Ltd of Changsha (Changsha, China). Animals were individually housed in SPF facilities during the experiment. All of the animal experimental protocols were approved by the Ethics Committee of Zunyi Medical University. All animal care and experimental procedures were performed in accordance with the Laboratory Animal Welfare and Ethics Committee of China.

Autophagy suppression model: Mice were injected with CTX (120 mg/kg) or Chloroquine (CLQ, 60 mg/kg) normal saline solution intraperitoneally to make autophagy suppression model. Mice were randomized into six groups with 10 mice in each group, including Normal control group (NC group), CTX group, CLQ group, CTX+CLQ group, ZgI (20mg/kg) + CTX+CLQ group and ZgI-AuNPs (20mg/kg, conaining ZgI 64.25μg) +CTX+CLQ group. Except the mice of NC group, mice in the other groups were injected with CTX or CLQ intraperitoneally. Mice in ZgI group and ZgI-AuNPs group were injected with 0.2mL ZgI and ZgI-AuNPs PBS solution via tail vein, respectively.

The CTX was injected one time at the first day of experiment. The CLQ was injected at the first, third and fifth day, respectively. The body weights and survival rate in each group were monitored daily for 6 days. At day 6 p.i., blood samples of each group were collected by retro-orbital puncture into heparinized tubes, the leucocyte and neutrophils were assayed by automatic blood cell counting instrument (URIT-3000, Shanghai, China). The bone marrow cell suspension was obtained by crushing mice bones (femurs and tibias) in PBS containing 2% FBS and filtering it through a 70mm cell strainer. Red blood cells were lysed by ammonium chloride solution to get the bone marrow cell suspension. As described earlier,¹⁸ Ckit⁺ cells were enriched for Ckit by MACS (MiltenyiBiotec) using anti-Ckit magnetic beads, and HSCs were sorted using a BD FACS Aria Fusion (BD Biosciences). The autophagy state of Ckit⁺ cells was observed by TEM.

Immunohistochemical Analysis

Mice HSCs were paraffin-embedded and sectioned, endogenous enzymes were inactivated, antigens were thermally repaired and the anti-P62 antibody was added (1:100 dilution) for 1 h at 37 °C. Then the anti-rabbit IgG (1:50 dilution) was added and incubated at 37 °C for 1 h. After that, the DAB was used for colour development, and the hematoxylin was used for restaining. Finally, the Image Pro Plus 6.0 image analysis software for semi-quantitative analysis of P62 expression in mice HSCs.

Statistical Analysis

All the data in this study were analyzed by SPSS 19.0. Data are expressed as mean ± SD. For values that were normally distributed, direct comparison between two groups was conducted by Independent Sample’s t-test. P value of < 0.05 was considered statistically significant.

Results

Characterization of ZgI-AuNPs

The preparation process of ZgI-AuNPs was briefly shown in Figure 3A, and the samples were characterized by particle size distribution, SEM, TEM, FTIR, DSC and XRD. From the Figure 3B we can see that the average particle size of ZgI-AuNPs is (340±16.5) nm, and the zeta-potential measurements showed that the produced ZgI-AuNPs had a charge of −35.49 ± 1.59 mV. In the water solution, the AuNPs had λ_max of absorption at 525 nm, while the ZgI-AuNPs had λ_max of absorption at 254 nm and 525 nm, respectively, preliminarily confirming the formation of ZgI-AuNPs (Figure 3C). In addition, the ZgI-AuNPs have an irregular cubic crystal structure (Figure 3D and E).

Figure 3 The preparation and characterization of ZgI-AuNPs. (A) The brief illustration of ZgI-AuNPs preparation. (B) Size distribution of AuNPs and ZgI-AuNPs. (C) Absorbance spectra of AuNPs and ZgI-AuNPs. (D) TEM image. (E) SEM image.

The FTIR analysis (Figure 4) showed that the characteristic peaks of ZgI are mainly at 3430, 2927, 1732, 1634, 1456, and 1073 cm⁻¹, corresponding to -OH stretching vibration, CH₂ antisymmetric stretching, C=O stretching vibration, C=C stretching vibration, CH₂ deformation vibration and the stretching vibration of C-O, respectively. Compared with the physical mixture, AuNPs and ZgI-AuNPs, there are two characteristic absorption peaks on the spectrum, which located at 1732 and 1456 cm⁻¹, respectively. The physical mixture mainly has absorption peaks at 3406, 2930, 1635, 1419, and 1052 cm⁻¹, corresponding to -OH stretching vibration, CH₂ antisymmetric stretching, C=C stretching vibration, CH₂ deformation vibration and C-O stretching vibration, respectively. Compared with the spectrum of ZgI, the physical mixture also has the characteristic absorption peak of ZgI around 1456 cm⁻¹, but the characteristic absorption peak at 1732 cm⁻¹ disappears. ZgI-AuNPs mainly have absorption peaks at 3443, 2921, 1634, and 1070 cm⁻¹. Compared with the spectra of ZgI and physical mixtures, the characteristic absorption peaks of ZgI-AuNPs at 1732 and 1456 cm⁻¹ disappear. The FTIR detection indicated that the ZgI had been loaded in AuNPs.

Figure 4 The FTIR spectrum. (a) Physical mixture. (b) ZgI-AuNPs. (c) ZgI. (d) AuNPs.

Figure 5 illustrates the DSC thermogram of ZgI-AuNPs in comparison with the thermograms of ZgI alone and AuNPs alone. The thermogram of ZgI alone has two distinct characteristic peaks at 140.41 and 187.10°C, respectively. The thermogram of AuNPs alone has one distinct characteristic peak at 224.72°C. The thermogram of physical mixture has two characteristic peaks at 159.34 and 217.99°C, respectively. The thermogram of ZgI-AuNPs has four characteristic peaks at 82.92, 148.65, 245.49 and 287.46 °C, respectively. The two characteristic peaks of the physical mixture are close to the characteristic peaks of ZgI and AuNPs alone, respectively, indicating that there are pure ZgI and AuNPs in the physical mixture. It is speculated that simple physical mixing cannot change the state of ZgI and AuNPs. In the spectrum of ZgI-AuNPs, it can be seen that its characteristic peaks not only appear near the characteristic peaks of ZgI and AuNPs, but also other peaks appear in the spectrum. It is speculated that the combination of ZgI and PEG-modified AuNPs caused the change of the peaks.

Figure 5 The DSC thermogram. (a) Physical mixture. (b) ZgI-AuNPs. (c) ZgI. (d) AuNPs.

The XRD patterns of AuNPs and ZgI-AuNPs are shown in Figure 6. The diffraction pattern of ZgI showed a significant number of peaks between 10 ° and 20 °, indicating that ZgI is crystalline. In conformity with the pure crystalline gold standard PDF card (PDF#99-0056), the pure AuNPs exhibit five distinctive peaks at 38.18°, 44.38°, 64.57°, 77.56°, and 81.72°, respectively. No other noticeable impurity peaks also emerged. The diffraction peaks of ZgI vanish from the spectrum, and the distinctive peaks of ZgI-AuNPs appear at 31.72°, 45.43°, 66.14°, 76.56°, and 84.11°, respectively, which correspond to the gold standard PDF card. It is assumed that ZgI-AuNPs were prepared successfully.

Figure 6 The XRD spectrum. (a) Physical mixture. (b) ZgI-AuNPs. (c) ZgI. (d) AuNPs.

Drug Loading Capacity

The drug loading capacity of ZgI loaded in the AuNPs were 160.63±1.35 μg·mg⁻¹ and the encapsulation efficiency of ZgI reached to (91.29±3.48)%. As shown in Table 1.

Table 1 The DL and EE of ZgI-AuNPss (n=3, mean±SD)

In vitro ZgI Release Test

Figure 7 showed the cumulative release rate of pristine ZgI and ZgI-AuNPs in water. Compared with the pristine ZgI, drug in AuNPs exhibted more slowly release rate, showing a sustained release. In the presented result we can see that approximate 100% of pristine ZgI was released at 10h, while the Zg I release in ZgI-AuNPs is only (56.30±1.97) % at the same point. At 48 h, the cumulative release of ZgI was (75.07±0.23) % for ZgI-AuNPs. The result suggested that ZgI-AuNPs exhibited a slower drug release rate than that of ZgI.

Figure 7 The test result of cumulative ZgI release. Data are presented as mean ± SD from three independent experiments.

Cytotoxicity in vitro

Cytotoxicity of CTX and ZgI-AuNPs were evaluated by the CCK-8 assay in vitro. The result showed that the effect of different concentrations of CTX on the survival rate of HSCS varies greatly. The survival rate of HSCS steadily declines as CTX concentration rises. According to Figure 8A, the survival rate of HSCS is near to 50% when CTX concentration is 0.125 μM. The best concentration to cause HSCS damage was therefore CTX at a concentration of 0.125 M. As demonstrated in Figure 8B, ZgI-AuNPs had no impact on the activity of HSCS in the concentration range of 5–160 μg·mL⁻¹, and all six experimental groups exhibited HSCS cell viability levels above 90%. The ZgI-AuNPs with a concentration of 5 μg·mL⁻¹ was finally selected as the optimal administration concentration.

Figure 8 The effect of ZgI-AuNPs on survival rate of HSCS. (A). The cytotoxicity of CTX in normal HSPC-1 cells (n = 3, Mean ± SD). (vs Normal, *P<0.05, **P<0.05). (B). The viability of HSPC-1 cells.

Effects on the Autophagy in HSCs in vitro

As shown in Figure 9A, TEM showed many autophagosomes, as shown by the yellow arrow, with a distinct double membrane structure in the normal group cells, but this structure was either absent in the cells of model group (Figure 9B), suggesting that the CTX weakened the autophagy in HSCs. A small number of autophagosomes can be observed in the AuNPs group cells (Figure 9C), and autophagosomes can also be observed in the cells of ZgI group (Figure 9D). However, there were significantly more autophagosomes in the cells of ZgI-AuNPs group compared with the cells of model, AuNPs and ZgI group (Figure 9E), indicating that the activity of ZgI promoting autophagy of HSCs was enhanced by gold nanoparticles.

Figure 9 The effect of ZgI-AuNPs on the autophagy of HSCS cells. The red labeling of “N” represents the cell nuclear, the “Mi” represents the mitochondria and the “RER” represents the rough endoplasmic reticulum. The yellow arrows represents the autophagosomes. (A).Normal group: saline + RPMI 1640. (B). Model group: CTX + RPMI 1640. (C). AuNPs group: CTX + AuNPs. (D). ZgI group: CTX + ZgI. (E). ZgI-AuNPs group: CTX +ZgI- AuNPs.

To further confirm the effect of gold nanoparticles on the ZgI, the LC3B, an important marker protein of autophagy was detected in HSCs of each group. According to Figure 10A, the fluorescence intensity of LC3B in the HSCs of the model group and ZgI group was significantly lower than that of the normal group (P < 0.01), indicating that the degree of autophagy in HSCs was greatly reduced following CTX injury. The fluorescence intensity of LC3B in the HSCs of the ZgI group was significantly increased (P < 0.05) when compared to the model group, and it was dramatically increased (P < 0.01) when ZgI-AuNPs administration was used. The fluorescence intensity of LC3B in the HSCs of the ZgI-AuNPs group was likewise considerably higher than that of the ZgI group at the same time (P < 0.05), demonstrating that AuNPs improved ZgI’s inhibitory effect on the decline in autophagy in HSCs brought on by CTX. Figure 10B displays the immunofluorescence detection of LC3B in the HSCS of each experimental group.

Figure 10 The effect of ZgI-AuNPs on the expression of LC3B in HSCS cells. (A).The mean of relative fluorescence density. (B)The green fluorescence of LC3B photographed by fluorescence microscope. Normal group: saline + RPMI 1640. Model group: CTX + RPMI 1640. AuNPs group: CTX + AuNPs. ZgI group: CTX + ZgI. ZgI-AuNPs group: CTX +ZgI- AuNPs. Data are presented as mean ± SD from six independent experiments (vs model group, *P<0.05, **P<0.05; vs AuNPs, ΔP < 0.05, ΔΔP < 0.01; vs ZgI, ★P < 0.05, ★★P < 0.01.).

Effects on the Autophagy and Hematopoietic Function of HSCs in vivo

In vivo experiments also confirmed that the ZgI-AuNPs significantly promoted autophagy in HSCs. From the Figure 11A we can see that a large number of autophagic lysosomes and autophagic vesicles are present in haematopoietic stem cells which maintain the normal haematopoietic function.Both the CTX and CLQ damaged autophagy in mice HSCs, inducing a significant decrease in autophagosomes and autophagic vesicles, as shown in Figure 11B and C. The autophagy of mice HSCs was more severely disturbed when CTX and CLQ were coupled, and autophagosomes and autophagic vesicles were hardly visible under transmission microscopy, as illustrated in Figure 11D. ZgI could partially protect mice HSCs from the autophagic degradation caused by CTX and CLQ, and few autophagosomes were seen in the ZgI group (Figure 11E). Numerous autophagosomes and autophagic vesicles were visible in mice HSCs treated with ZgI-AuNPs, which was in line with our anticipated outcome (Figure 11F). As a result, it was discovered that the gold nanoparticles enhanced ZgI’s ability to promote autophagy in mouse HSCs.

Figure 11 The effect of ZgI-AuNPs on the autophagy of mice HSCs cells. (A).NC group: saline. (B). CTX group:CTX + saline. (C). CLQ group: CLQ + saline. (D). CTX+CLQ group: CTX + CLQ + saline. (E). ZgI group: ZgI + CTX + CLQ + saline. (F).ZgI-AuNPs group: ZgI-AuNPs + CTX + CLQ + saline. The yellow arrows represents the autophagosomes.

Additionally, the immunohistochemical assay showed that P62 protein was significantly upregulated in mice hematopoietic stem cells after CTX and CLQ injection when compared with the normal group (Figure 12a), particularly in mice treated with the combination of CTX and CLQ, as shown in Figure 12b–d, respectively, suggesting that CTX and CLQ caused inhibition of autophagy in mice hematopoietic stem cells. Following ZgI and ZgI-AuNPs treatment, the P62 protein expression was considerably reduced in hematopoietic stem cells from mice, and ZgI-AuNPs-treated mice displayed a higher reduction in P62 protein expression than ZgI-treated mice, as shown in Figure 12e and f. It is suggested that ZgI can restore the autophagic destruction of hematopoietic stem cells induced by CTX and CLQ, and the gold nanoparticles can further enhance the autophagy-promoting effect of ZgI on hematopoietic stem cells.

Figure 12 The immunohistochemical detection of P62 protein in haematopoietic stem cells. (A) The immunohistochemical images of P62 protein. (B) The statistical chart of P62 protein-positive cells. (a).NC group: saline. (b). CTX group:CTX + saline. (c). CLQ group: CLQ + saline. (d). CTX+CLQ group: CTX + CLQ + saline. (e). ZgI group: ZgI + CTX + CLQ + saline. (f).ZgI-AuNPs group: ZgI-AuNPs + CTX + CLQ + saline. Data are presented as mean ± SD from six independent experiments (vs CTX+CLQ group group, *P<0.05, **P<0.05; vs ZgI, ΔΔP < 0.01).

Finally, we assessed the survival rate, body weight, and quantity of leukocytes and neutrophils in the peripheral blood of each experimental group to further support the beneficial effect of autophagy on the hematopoietic capacity of mice HSCs. Figure 13A and B show that mice injected with both CTX and CLQ experienced rapid weight loss starting on day three (P<0.05), and that the mortality rate significantly increased starting on day two, showing that mice are vulnerable to passing away when autophagy of HSCs is seriously disrupted. Leukocyte and neutrophil counts in the peripheral blood of mice given injections of CTX and CLQ were similarly dramatically decreased (P<0.01), as demonstrated in Figures 13C and D.

Figure 13 The effect of ZgI-AuNPs on the blood cell production capacity of mice HSCs. (A).The survival rate of each group. (B)The body weight change of each group (n = 6, Means ± SD), (vs CTX group, *P<0.05, **P<0.05; vs CTX+CLQ group, ΔP < 0.05, ΔΔP < 0.01). (C).The leukocytes count of each group (n = 6, Means ± SD), (vs CTX group, *P<0.05, **P<0.05; vs CTX+CLQ group, ΔP < 0.05, ΔΔP < 0.01; vs ZgI group, ■P < 0.05). (D) The neutrophils count of each group(n = 6, Means ± SD), (vs CTX group, *P<0.05, **P<0.05; vs CTX+CLQ group, ΔP < 0.05, ΔΔP < 0.01; vs ZgI group, ■P < 0.05). NC group: saline. CTX group:CTX + saline. CLQ group: CLQ + saline. CTX+CLQ group: CTX + CLQ + saline. ZgI group: ZgI + CTX + CLQ + saline. ZgI-AuNPs group: ZgI-AuNPs + CTX + CLQ + saline.

It suggests that mice HSCs’ capacity to create leukocytes will be significantly reduced if their autophagy is eliminated. When mice were treated with ZgI-AuNPs, their body weight was well maintained (P<0.05), mortality was significantly reduced, and the number of leukocytes (P<0.01) and neutrophils (P<0.01) in peripheral blood was also significantly increased, and the above treatment effects were significantly better than those of ZgI treatment (P<0.05). It suggests that ZgI-AuNPs can effectively counteract the decline in HSCs’ hematopoietic potential brought on by CTX and CLQ. At the same time, ZgI’s hematopoietic effect on mice HSCs is strengthened by the gold nanoparticles.

Discussion

CTX is the most widely used alkylating agent antitumor drug in clinic, and it is also an immunosuppressant, which is often used in the treatment of various malignant tumors such as leukemia, lymphoma and breast cancer.¹⁹ In mice, CTX drastically decreased the number of blood cells, and it was initially verified that this was due to the damage CTX caused to the HSCS. This experimental investigation discovered that one key mechanism of CTX’s harm to the body’s hematopoietic system may be the loss of autophagy in HSCS produced by CTX. After excessive DNA damage from CTX’s alkylation of cellular DNA or RNA, which impairs the ability of cells to repair themselves, autophagy is lost, which reduces the capacity of HSCS to self-renew and differentiate, which results in the development of hematological diseases. Further research is still needed to determine how exactly CTX suppresses autophagy in HSCS.

HSCs are the adult stem cells of the blood system that have the capacity for long-term self-renewal and the potential to differentiate into various types of mature blood cells.²⁰ Approximately one third of adult HSCs have a strong long-term regenerative potential similar to that of healthy young HSCs, exhibiting high levels of autophagy and a low metabolic state.²¹ Autophagy inhibits HSCs metabolism by removing active, healthy mitochondria, maintaining homeostasis and stemness, and maintaining the regenerative capacity of older HSCs becomes increasingly important as we age.²² Therefore, when the normal autophagy of HSCs is disrupted, the hematopoietic function of HSCs will be weakened and even cause the death of HSCs. In our study, we cannot observe autophagy in CTX and CLQ treated mice HSCs. The loss of autophagy in HSCs depletes leukocytes in the peripheral blood of mice and is also lethal to mice. The ZgI-AuNPs has a very significant protective and promotive effect on autophagy in mice HSCs, protecting them from destruction by CTX and CLQ and thus restoring the hematopoietic function of HSCs.

In brief, we have successfully formulated SH-PEG-NH₂-coated gold nanoparticles loading Ziyuglycoside I with a sustained drug release and enhanced autophagy of HSCs. Therefore, AuNPs can serve as ZgI carriers for the promotion of ZgI transcellular uptake and enhancement of pro-autophagy activity.

Conclusion

In this paper, we successfully developed a SH-PEG-NH₂-coated gold nanoparticles loading Ziyuglycoside I, which aimed to enhance the activity of ZgI promoting the autophagy of HSCs. The prepared ZgI-AuNPs have an irregular cubic crystal structure with an average particle size of (340 ± 16.5) nm. And the AuNPs can load the ZgI at a content of 160.63 ± 1.35 μg·mg⁻¹. Meanwhile, the AuNPs can reduce the drug release rate of ZgI. The current study also showed the ZgI-AuNPs roles in promoting the autophagy of HSCs both in vitro and in vivo and enhancing the hematopoietic effect of ZgI on mice HSCs.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Funding

This work was supported by the National Natural Science Foundation of China (NO. 82260723) and the Science and Technology Support Program of Guizhou province (NO.[2021]Genaral410).

Disclosure

The authors declare no conflict of interest.

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