Anti-Inflammatory Activation of Phellodendri Chinensis Cortex is Mediated by Berberine Erythrocytes Self-Assembly Targeted Delivery System
Introduction
Phellodendri Chinensis Cortex (PCC, Figure 1), known as “Chuan HuangBai” in China, is a commonly used Chinese medicinal herb derived from the dried bark of Phellodendron chinense Schneid.1 PCC has the function of clearing heat, eliminating dampness, purging fire, and removing toxicity, and is traditionally applied in the treatment of inflammatory diseases.2,3 Protoberberine alkaloids are regarded as the major active components of PCC. Particularly, berberine (BBR, Figure 1), the most predominant protoberberine alkaloid and one of the quality control markers of PCC, is largely accountable for the potential therapeutic effects of PCC. BBR has been found to possess appreciable therapeutic activities in inflammation-related disorders and illnesses such as inflammasome-involved kidney injury,4 ulcerative colitis,5 and osteoarthritis.6
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Figure 1 The relationship between Phellodendri Chinensis Cortex (PCC), berberine (BBR) and oxyberberine (OBB). |
However, the extremely low plasma concentration and oral bioavailability (0.36%) of BBR are insufficient in experimental or clinical settings to yield the effects as observed under experiments, which raises challenges to adequately explain its exceptional anti-inflammatory potency in clinical trials.7,8 The majority of researches on BBR metabolism were concentrated on the intestinal tract and hepatic system. Plenty of pharmacokinetic-pharmacodynamic studies on BBR are concentered on its concentration and metabolites in plasma; however, it was not sufficient to explain the contradiction between its low plasma concentration and high potency.
Erythrocyte metabolism is an important metabolic pathway that is easily ignored. Erythrocytes are the main cells in blood circulation, which provide the extraordinary vehicles for the dissemination of drugs in blood circulation.9 Hemoglobin (Hb), the most abundant protein in the blood, possessed the properties of catalase thus catalyzing the oxidation reaction.10,11 In our recent work, we have found that BBR could bind to Hb assembly and form a BBR-erythrocytes drug delivery system.12 Furthermore, oxyberberine (OBB, Figure 1), whose C-9 position is oxidized to a carbonyl group to form an amide ring with 8-N atoms, has been identified as the essential metabolite after intravenous administration of BBR in our previous study.13 Erythrocytes are equipped with various reactive oxygen species and enzymes to catalyze drug metabolism, particularly drug oxidation.14,15 Furthermore, erythrocytes identify the drug molecules and deliver them to the target site, resulting in the hepatic accumulation.16 Herewith, erythrocytes have currently been deemed as the excellent site-targeted delivery system.17,18 However, the potentially important role that erythrocytes play in the metabolism and disposition of BBR may have been ignored.
On the basis of our preliminary study, erythrocytes are hypothesized as the other metabolic site of BBR besides intestinal tract and liver. As part of our ongoing search and to test our hypothesis, the present endeavor has been devoted to exploring the role of erythrocytes in the metabolism and delivery of BBR to reveal the anti-inflammatory mechanism of PCC. Our study furnished a novel version to interpret the paradox between excellent anti-inflammatory effect and extremely low absolute bioavailability of BBR from the perspective of the erythrocyte-drug delivery system and the quantitative-effect relationship. The results will contribute to a better understanding of the underlying material basis of the anti-inflammatory effects of BBR and PCC, and are also envisaged to provide a paradigm for erythrocytes in drug metabolism and provide an enlightenment for the study on activity presentation of Chinese medicine from the erythrocyte drug carrier system.
Materials and Methods
Chemicals and Reagents
PCC and berberine hydrochloride (purity >98%) were provided by Guangzhou Herbal Medicine Company (Guangzhou, China) and Chengdu HerbPurity CO., Ltd. (Chengdu, China), respectively. OBB (purity >98%) was synthesized and identified following our preceding study.19 High-performance liquid chromatography (HPLC) – grade acetonitrile was bought from Guangzhou Lubex Biological Technology Co., Ltd. (Guangzhou, China). Trifluoroacetic acid (TFA) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Deionized distilled water was supplied by China Resources C’estbon Beverage (China) Co., Ltd. Lipopolysaccharide (LPS), dimethyl sulfoxide (DMSO) and Evans blue were obtained from Sigma-Aldrich (St. Louis, MO, USA). Enzyme-linked immunosorbent assay (ELISA) kits (TNF-α, IL-6, and IL-18) were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). BCA protein assay kit was obtained from Shanghai BestBio Biological Technology Co., Ltd. (Shanghai, China). Other reagents were of the highest purity and analytical grade.
Preparation of PCC Extract
The PCC extraction method was employed according to Wang et al with some modifications.20 PCC was crushed into powder (300 g) and extracted with 1200 mL 80% ethanol (1:4, w/v) solution at 75 °C for 3 h, which was performed three times. The PCC extract was then filtered and merged for rotatory evaporation till no alcohol taste was found. Finally, the residue was suspended in 0.9% saline. BBR content was measured by HPLC (LC-20AT, Shimadzu, Kyoto, Japan), and the final concentration of BBR was 40 mg/mL.
Animals
Male Sprague Dawley (SD) rats (220–240g) and Kunming mice (18–22g) of both sexes were purchased from the Medical Experimental Animal Center of Guangzhou University of Chinese Medicine. The experimental procedures were conducted under the approval of the Institutional Ethics Committee of Guangzhou University of Chinese Medicine (Ethics No. 20210609001) and carried out in accordance with the Guidelines on the Care and Use of Laboratory Animal issued by the Chinese Council on Animal Research and the Guidelines of Animal Care. Animals were housed under specific pathogen-free conditions of 22 ± 2 °C and 55 ± 2% humidity with a 12-h light/dark cycle, and fed with conventional food and water ad libitum.
Pharmacokinetic Study
For the analysis of pharmacokinetics of PCC in vivo, six SD rats were orally administered with a single dose of PCC extract, which corresponded to an equivalent dose of BBR (100 mg/kg) via intragastric administration. The blood was collected from the orbital sinus venous plexus of rats in the sampling vessel which was infiltrated with heparin sodium at 0.083, 0.167, 0.25, 0.5, 1, 2, 2.5, 4, 5, 6, 7, 8, 12, and 24 h.
The blood samples were treated according to our previous study with some modifications.13 The whole blood stood at 4 °C for 2 h and was then centrifuged at 2500 rpm for 10 min to obtain plasma from upper layer and erythrocytes from lower layer. And, 250 μL of plasma was mixed with the internal standard (I.S.), precipitated by 1 mL acetonitrile, and subsequently centrifuged at 8000 rpm for 10 min at 4 °C. The supernatant was collected, dried in an electric oven, and redissolved with 200 μL methanol. Erythrocyte samples were washed with normal saline (1:5, v/v) three times mixed with 15% HCl and allowed to react at 60 °C for 6 h to hydrolyze the protein. After centrifugation at 8000 rpm for 10 min, the supernatant was loaded into the pre-treated SPE column and eluted with three times of methanol. The eluents were concentrated and redissolved in 200 μL acetonitrile. All the samples were filtered through a 0.22-μm filter membrane before HPLC analysis.
HPLC Analysis
HPLC system (LC-20AT, Shimadzu, Kyoto, Japan) was used to determine the concentrations of BBR and OBB in the plasma and erythrocytes. Phenomenex C18 column (250 × 4.6 mm, 5 μm) was employed for separation analysis. The mobile phases were consisted of solvent A (water with 0.1% trifluoroacetic acid) and solvent B (acetonitrile). The gradient elution settings were as follows: 90–10% A for 0–20 min, and 10–90% A for 20–30 min. The UV detection wavelength was 345 nm. Then, 10 μL samples were injected for determination, and the flow rate was 1.0 mL/min.
Method Validation
Selectivity was determined to detect the interferences of analytes and piperine (internal standard, IS) at the retention time by evaluating blank whole blood samples from six rats as well as whole blood samples spiked with analytes. Linearity was determined by plotting the peak area ratio of analytes/IS against the concentrations of analytes in plasma and erythrocytes through least-squares linear regression using 1/x2 as a weight factor. The lower limit of quantification (LLOQ) was defined as the lowest concentration with a signal-to-noise ratio of at least 10.
The accuracy, precision, and recovery of the method were tested with the quality control (QC) samples prepared within the calibration range. Six replicates of each QC sample were determined to acquire the mean concentrations and then compared with the nominal concentrations to evaluate the accuracy, inter- and intra-day precision. The stability of samples was evaluated after storage at room temperature for 4 h, at −10 °C for 1 month, and after three freeze–thaw cycles from −80 °C to room temperature. The relative recovery of each analyte was evaluated by comparing the average peak areas of the QC samples after extraction with the average peak areas of spiked-after extraction samples, representing the 100% recovery value.
Bioconversion of BBR by Erythrocytes in vivo
BBR was dissolved in normal saline for injection. The BBR solution was intraperitoneally administered to the rats at a volume of 2.5 mg/kg body weight. Whole blood was collected from the fundus venous plexus at 0.5, 1, and 2 h after administration and treated according to Pharmacokinetic study as mentioned above.
Incubation of BBR and OBB in vitro
The in vitro study was performed to further explore the role of erythrocytes on the transportation and existing form of BBR. BBR and OBB were added to the blank whole blood and plasma samples respectively to obtain a final level of 10 μM. Post incubation at 37 °C for 2 h, 4-fold acetonitrile was added to the incubation system to terminate the reaction and then manipulated following Pharmacokinetic study as described above. In addition, another whole blood sample was hydrolyzed by HCl and then treated according to Pharmacokinetic study as mentioned above. Finally, the contents of BBR and OBB in whole blood and plasma samples were comparatively quantified using HPLC.
Routine Blood Test: Assessing the Relationship Between the Drug Concentration in Erythrocytes and the Amounts of Hemocytes
The blood test was performed as described in the literature21 with minor modifications. The amounts of erythrocyte, leukocyte, lymphocyte, neutrophil, monocyte, and Hb were counted and recorded.
Tissue Distribution Mediated by Erythrocytes
SD rats divided into four groups (n = 6) were orally administered a single dose of 400 mg/kg BBR and sacrificed at 1, 2, 4, and 6 h respectively after being anesthetized by ether. Afterwards, the liver, kidney, heart, spleen, lung, intestine, pancreas, brain, and muscle were removed rapidly, washed with normal saline, cut into small pieces, and dried with filter paper. Then, 0.25 g of these samples were put into the centrifuge tubes and homogenized with a homogenizer (Wuhan Servicebio Technology Co., Ltd.) with the addition of acetonitrile at a ratio of 1:4 (w/v). Centrifugation was conducted at a speed of 8000 rpm for 10 min, and the supernatant was dried in an electric oven, redissolved with 200 μL methanol and filtered with 0.22-μm nylon membrane prior to injection into HPLC.
Immunohistochemical Staining: Assessing the Relationship Between the Tissue Distribution and the Amounts of Macrophages
Immunohistochemistry was used to characterize the macrophages (CD68, dilution 1:500, Servicebio, Wuhan, China). Specimens of the normal rat tissues (liver, spleen, kidney, heart, lung) were fixed in 4% neutral formalin solution and then routinely processed as previously described22 with minor modifications. Under the observation at high magnification (×400) from five randomly selected sampling areas, CD68+ macrophages were quantified using images captured with a digital camera system and calculated by using Image-Pro Plus software (Media Cybernetics, MD, USA). Results were expressed as the average number of CD68+ cells per image.
Alteration of Anti-Inflammatory Activity of BBR
Cell Culture and Treatment
The murine macrophage RAW 264.7 cells were purchased from the American Type Culture Collection (Rockville, MD, USA). Cells were incubated in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a humidified incubator with an atmosphere consisting of 95% air and 5% CO2 at 37 °C until 70–80% confluency before drug treatment. RAW 264.7 cells were pre-treated with BBR or OBB for 4 h and subjected to stimulation of LPS for another 24 h.
Cell Viability Assay
RAW 264.7 cells were seeded into the 96-well plate (1×105 cells/well, 100 μL medium/well) for 24 h. Cells were treated with BBR (80, 40, 20, 10, 5, 2.5 μM), or OBB (80, 40, 20, 10, 5, 2.5 μM) with or without LPS. After incubation at 37 °C for 24 h, 10 μL cell counting kit-8 solution was added to each well and incubated for 0.5 h. The absorbance was detected at 450 nm by a microplate analyzer.
ELISA for TNF-α, IL-6, and IL-18 Levels
Cells were seeded into 12-well plate (1×105 cells/well, 500 μL medium/well) and pre-treated with OBB (0.1, 0.2, 0.4 μM) and BBR (5 μM) for 4 h, then treated with LPS (1 μg/mL) for an additional 24 h. The supernatant was extracted from each well for the ELISA test kits to determine the content of inflammatory cytokines according to the manufacturer’s instructions. The dosages of BBR and OBB were employed based on our prior trial.
Acetic Acid-Induced Vascular Permeability Test
The assay was performed according to the previous study23 with some modifications. During the experiment, mice were randomly divided into six groups (5 male and 5 female per group). The treatment groups were orally administered with different doses of OBB (1, 2, 4 mg/kg) and BBR (4 mg/kg, positive drug) for 7 consecutive days. The normal group and the model group were given normal saline as a control. One hour after the last administration, mice were intravenously injected with 1% Evans blue in saline (10 mL/kg) and then given an intraperitoneal injection of 0.6% (v/v) acetic acid (10 mL/kg). Mice were sacrificed after 20 min. The abdominal cavity was washed with 5 mL ice-cold normal saline. The peritoneal exudate was collected after centrifuged at 3000 rpm for 10 min. The absorbance of supernatants was measured at 590 nm using a SHIMADZU UV2600 UV–VIS spectrophotometer (Shimadzu, Tokyo, Japan). The vascular permeability was evaluated by quantifying the infiltration of Evans blue dye into the peritoneal cavity.
Statistical Analysis
Data are expressed as mean ± standard error of the mean (S.E.M.). Shapiro–Wilk test was applied to assess the normal distribution of the data. Student’s t-test or One-way ANOVA/Dunnett’s t-test was used for the assessment of significance between two groups or multiple groups. Statistical analysis was performed by SPSS software (version 26.0; SPSS Inc, Chicago, IL, USA). Values of p < 0.05 or p < 0.01 were considered statistically significant.
Results
Preparation of PCC Extract
As shown in Figure 2, the content of BBR in PCC was over 3.0%, which met the requirement for the extract of PCC according to Chinese Pharmacopeia (2020 edition). OBB in PCC extract was beyond the limit of detection (LOD) of HPLC.
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Figure 2 BBR in PCC extract was analyzed using HPLC. (S1) PCC extract with 95% ethanol. The purity of BBR was over 95% and the content of BBR was over 3.0%. (S2) BBR standard solution. |
HPLC Method Validation
The above HPLC method was validated for simultaneous quantitative detection of BBR and OBB in rat plasma and erythrocytes. Some representative HPLC images of samples are shown in Figure 3. Under this developed method, BBR, OBB, and IS can be separated well from each other and no endogenous substances affected the detection of the target peaks. The HPLC chromatogram indicated that OBB was the metabolite of BBR rather than the chemical oxidative product of BBR. Calibration curves were found to be linear over the concentration ranging from 0.049 to 12.500 μg/mL for BBR and OBB, both with correlation coefficient over 0.9995.
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Figure 3 HPLC chromatograms of blood samples with BBR and OBB. (S1) Blank whole blood treated with acetonitrile precipitation. (S2) Blank whole blood hydrolyzed by HCl. (S3–S5) Whole blood spiked with BBR, OBB, and IS standard solutions. (S6) Plasma samples obtained following intraperitoneal injection of BBR in 0.5 h. (S7) Erythrocyte samples acquired after intraperitoneal administration of BBR in 0.5 h. (S8) BBR hydrolyzed by HCl. |
The accuracy and precision for the measurement of BBR and OBB in rat plasma and erythrocytes are shown in Table 1. The relative errors (RE%) of accuracy were within 15%, and the relative standard deviations (RSD%) of intra-, inter-day precision remained within 15%. The recovery rates were in the range of 85–115%. As tabulated in Table 2, RSD% of stability of the analytes was lower than 15%, indicating that samples were stable during storage at room temperature for 4 h, at −10 °C for 1 month and following three freeze–thaw cycles from −80 °C to room temperature, respectively. Therefore, the linearity, accuracy, precision, recovery, and stability of biological samples satisfied the standards for quantitative analysis.
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Table 1 Accuracy, Recovery, and Intra-Day and Inter-Day Precisions of BBR and OBB in Plasma and Erythrocytes (n = 6) |
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Table 2 The Stability (RSD %) of BBR and OBB in Plasma and Erythrocytes Under Three Different Storage Conditions (n = 6) |
Pharmacokinetic Study
As shown in Figure 4A, post oral administration of PCC, the Tmax of BBR in rat blood was 1 h and subsequently maintained at a low plasma concentration for 24 h. Of note, compared to BBR in plasma, the area under the blood drug concentration–time curves (AUC0-t) and the peak concentration (Cmax) for the concentration of BBR in erythrocytes were dramatically elevated (p < 0.01). It was interesting to find that OBB was detected after administration of PCC. Figure 4B indicates that AUC0-t and Cmax of OBB in plasma were both much lower than those in erythrocytes. Either in plasma or erythrocytes, the concentration and AUC0-t of OBB were lower than those of BBR, which indicated OBB was presented at a merely low concentration and the major constituent of PCC in vivo was BBR. The second concentration peak of OBB appeared 3 h later than BBR, suggesting that erythrocytes might perform a potential role in retarding drug release.
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Figure 4 Blood concentration–time curves of BBR and OBB post oral administration of PCC. Blood concentration–time curve of BBR (A) and OBB (B). Data are expressed as mean ± S.E.M. (n = 6). |
Determination of BBR and OBB in vitro
To explore the effect of erythrocytes on the existing forms of BBR and OBB, different methods were used to treat the blood samples incubated with BBR/OBB. As tabulated in Table 3, in comparison to the plasma sample, the concentrations of BBR/OBB in whole blood were significantly decreased after treatment with acetonitrile. The result indicated that BBR entered into the erythrocytes, and BBR and OBB bound with Hb. The levels of BBR and OBB in the whole blood treated with HCl were significantly higher than those treated with acetonitrile since Hb was hydrolyzed and the drugs were released.
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Table 3 The Concentration of BBR and OBB in Plasma and Whole Blood After Incubation in vitro in 2 h (n = 6) |
Effect of Erythrocytes on the Metabolic Feature of BBR
Concentrations and partition coefficients of BBR and OBB in plasma and erythrocytes were revealed in Figures 5 and 6, respectively. The concentration of BBR was 0.067 ± 0.031, 0.049 ± 0.032, 0.025 ± 0.025 μM in plasma respectively and 0.073 ± 0.044, 0.154 ± 0.068, 0.160 ± 0.033 in erythrocytes respectively at 0.5, 1 and 2 h. The partition coefficient between plasma and erythrocytes (Kp/b) of BBR and OBB showed a downward trend, indicating that both BBR and OBB in plasma decreased gradually and had an opposite tendency in erythrocytes. OBB was detected after intraperitoneal injection of BBR and the proportion of OBB in erythrocytes was 13.5%, 23.32%, and 46.24% respectively at 0.5, 1 and 2 h, indicative of an increasing trend in rats in vivo.
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Figure 5 (A) Concentrations of BBR and its metabolite OBB measured in rats after intraperitoneal injection of BBR at the dose of 2.5 mg/kg body weight at 0.5, 1, and 2 h. (B) Trend variation of plasma/erythrocytes partition coefficients of BBR and OBB in vivo at 0.5, 1 and 2 h. Data are expressed as mean ± S.E.M. (n = 6). |
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Figure 6 The proportion of BBR and OBB in whole blood after intraperitoneal injection of BBR at the dose of 2.5 mg/kg at 0.5, 1, and 2 h. |
Relationship Between the Drug Concentration in Erythrocytes and the Amounts of Monocytes
The changes in the number of rat hemocytes after intraperitoneal injection of BBR at 0.5, 1, 2 h were shown in Figure 7. The amounts of erythrocytes had no significant changes during this period (Figure 7A). Total leukocyte count and Hb had a significant reduction at 2 h (Figure 7B and C) and the number of monocytes was found to decrease markedly in 1 and 2 h (Figure 7C) compared to those of the normal group. Pearson correlation analysis showed that the numbers of monocytes in the peripheral blood were highly related to the concentrations of BBR and OBB in erythrocytes (r = −0.970, p = 0.030).
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Figure 7 Routine blood tests in rats after intraperitoneal injection of BBR at 0.5, 1, and 2 h at the dose of 2.5 mg/kg body weight. Changes in numbers of erythrocytes (A), Hb (B), and immune cells (C). (D) Significant correlations between drug concentration in erythrocytes and the amounts of monocytes. Data are presented as mean ± S.E.M. (n = 3). *p < 0.05 vs the normal group. |
Tissue Distribution
As shown in Figure 8, BBR was found to spread throughout the body including liver, kidney, heart, spleen, lung, intestine, pancreas, brain, and muscle within 6 h. BBR had a maximum concentration in the intestine, followed by the liver, indicating that BBR was absorbed from the intestine into the blood and mainly transported to the liver then to other organs via systemic circulation. OBB, a metabolite of BBR, was found in the liver, kidney, heart, lung, intestine, and pancreas, and was barely detectable in the brain and muscle after oral administration of BBR. OBB was transported to various target organs via erythrocytes after absorption, and the liver was the main target organ.
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Figure 8 Distribution profiles of BBR and OBB in the liver (A), spleen (B), heart (C), lung (D), kidney (E), intestine (F), brain (G), muscle (H), and pancreas (I) in rats post oral administration of 400 mg/kg BBR at 1, 2, 4, and 6 h. Data are presented as mean ± S.E.M. (n = 6). |
Evaluation of Immunohistochemistry
The numbers of CD68+ macrophages in different tissues were yielded by averaging the quantities of positively stained cells at high magnification (× 400) from each image. Of these five tissues, the spleen possessed the largest number of CD68+ macrophages, followed by the liver, lung, heart, and the least abundance of CD68+ cells was present in the kidney (Figure 9A). However, the concentrations of BBR and OBB were the highest in the liver and nearly the lowest in the spleen within 6 h (Figure 9B). The levels of OBB and BBR in tissues were not linear simply to the number of CD68+ macrophages.
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Figure 9 (A) The amount of CD68+ macrophages in different tissues of normal rats (n = 3). (B) The concentrations of BBR and OBB in tissues at 1, 2, 4 and 6 h (n = 6). Data are presented as mean ± S.E.M. |
Evaluation of the Anti-Inflammatory Effect of BBR and Its Metabolite OBB
Cytotoxic Effect of OBB and BBR on RAW264.7 Macrophages
To assess the potential cytotoxic effect of BBR and OBB on RAW 264.7 cells, diverse concentrations of BBR (2.5, 5, 10, 20, 40, 80 μM) and OBB (2.5, 5, 10, 20, 40, 80 µM) with or without LPS were used to incubate the cells for 24 h. As shown in Figure 10, the CCK8 assay indicated that OBB exhibited no significant inhibition on the growth of RAW 264.7 cells in the concentration ranging from 2.5 to 80 μM. However, BBR up to the concentration of 80 μM showed toxic effect on RAW 264.7 cells in parallel to the control cells. Besides, co-treatment with OBB and LPS showed no significant toxicity. The combination of BBR and LPS produced significant toxicity when the concentration of BBR reached above 40 μM.
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Figure 10 Cell viability of RAW 264.7 macrophages incubated with OBB and BBR with or without LPS (1 μg/mL) for 24 h. Cells were exposed to different drugs: (A) OBB, (B) OBB and LPS, (C) BBR, (D) BBR and LPS. The cell viability was determined by CCK8 assay. Data are presented as mean ± S.E.M. (n = 3). ##p < 0.01 vs the control group. |
Inflammatory Cytokine Production in RAW 264.7 Macrophages
As depicted in Figure 11A–C, stimulation of LPS upregulated dramatically the contents of pro-inflammation cytokines TNF-α, IL-6 and IL-18 in comparison to the control counterpart. OBB (0.1, 0.2 and 0.4 μM) significantly suppressed the levels of TNF-α, IL-6, and IL-18. Of note, RAW 264.7 cells treated with 0.4 μM OBB resulted in significantly decreased levels of IL-6 and IL-18 as compared to those treated with BBR (5 μM). These results indicated that OBB might exert superior anti-inflammatory effect to BBR even at a low dose.
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Figure 11 Comparison of anti-inflammatory effects between BBR and OBB in vitro and in vivo. (A–C) Effect of OBB on secretions of pro-inflammatory mediators TNF-α, IL-6, and IL-18 in LPS-induced RAW 264.7 macrophages. (n = 3). (D) Vascular permeability in mice induced by acetic acid. The capillary permeability was described by the content of Evans blue expelled into peritoneal cavity, which was measured by the OD of the supernatant (n = 10). Data are presented as mean ± S.E.M. &&p < 0.01 vs the Control group; ##p < 0.01 vs the Model group; *p < 0.05 vs the BBR group. |
Acetic Acid-Induced Vascular Permeability in Mice
As presented in Figure 11D, pretreatment with OBB (1, 2, 4 mg/kg) and BBR (20 mg/kg) significantly suppressed the vascular permeability in a dose-dependent manner compared to the model group. The inhibitory rates of OBB (1, 2, 4 mg/kg) and BBR (20 mg/kg) were 11.82%, 25.97%, 33.34% and 26.28%, respectively. The inhibitory effect of OBB at the dose of 2 mg/kg was as effective as that of BBR at the dose of 20 mg/kg.
Discussion
Berberine is a renowned alkaloid that has been documented to have various pharmacological activities. Due to the contradiction between its therapeutic effect and limited plasma concentration, BBR is always a hot issue in its biological and pathological processes. In terms of pharmacodynamics, the limited studies available mainly focused on the level of components in plasma,20 while the study on the role of other major components in blood like erythrocyte is rare and insufficient. Erythrocytes, the most abundant blood cell type, occupy a quarter of the total cell numbers within the human body.24 Erythrocytes harbor various distinctive properties such as unmatched long circulation, biocompatibility and biodegradability that make them appealing vascular carriers for in vivo delivery of natural and synthetic payloads.9,25 Although our previous study has suggested a BBR erythrocytes-Hb self-assembly delivery system,12 the metabolic aspect, targeted delivery and dose–effect relationship of BBR mediated by erythrocytes have not yet been clearly clarified.
It has been reported that BBR-erythrocytes delivery system has been developed artificially and erythrocytes served as delivery vehicles for BBR to achieve the long-acting hypolipidemic effect.26 In our previous work, a BBR erythrocytes-Hb self-assembly delivery system has been identified and the accumulation of BBR in erythrocytes was attributed to the intense conjugation of BBR with Hb.12 In this study, BBR achieved a significantly higher concentration in erythrocytes than in plasma in rats after intragastrical administration with PCC and intraperitoneal administration with BBR, which was in agreement with our previous findings.12,13 HCl, a hydrolyzing agent, could be used for the hydrolysis of drug-Hb adducts.27 Incubation samples of BBR and whole blood (including plasma and erythrocytes) treated with HCl have been found to have a notable increase in BBR concentration. This observation indicated that BBR was present predominantly in erythrocytes and released from the Hb complex. Hence, erythrocytes, acting as the hidden carriers, could autonomously load and deliver BBR.
BBR has limited solubility and low absorption in the small intestine.28 It has been acknowledged that an oral dose of 400 mg/kg BBR resulted in a maximum plasma concentration (Cmax) of 0.4 ng/mL.29 And the absolute bioavailability of BBR was less than 1%.30,31 The contradiction between the explicit therapeutic effects of berberine and its very low plasma concentration promotes the hypothesis that the metabolites of berberine may substantially contribute to its biological activities.32 As reported, 43.8% of BBR was metabolized7 and it could be metabolized into 97 metabolites in rat,33 which explained to some extent its low plasma concentration in vivo. However, studies on BBR metabolites have mainly concentrated on the common and easily found metabolites in the liver, such as berberrubine, demethyleneberberine, palmatine, jatrorrhizine and thalifendine,20,34–37 the pharmacokinetic profiles of other metabolites remained ambiguous.
In addition to functioning as a drug carrier, erythrocytes also serve as metabolic site. Erythrocyte metabolism, independent of hepatic and gastric metabolism, is a frequently overlooked metabolic pathway. Erythrocytes are the transporters of oxygen and contain reactive oxygen species which may cause oxidative metabolism of drugs.14,38 Similarly, the accumulation of hydrogen peroxide in erythrocytes was considered to account for the formation of a metabolite. Furthermore, Hb was expected to achieve catalytic reactions with selectivity from the interaction of the substrate with a natural, evolvable binding site.39,40 However, the metabolic function of erythrocytes for drugs has not received sufficient attention. In our previous work, OBB, one of the metabolites of BBR, has been found to be transformed from BBR via erythrocyte metabolism either in vivo or in vitro in our previous work.41 Therefore, in addition to metabolism in the liver and intestine, erythrocytes might perform an important role in the metabolism of BBR by predominating oxidative reaction pathway.
OBB has been reported to possess superior anti-arrhythmic, antifungal and antitumor activities to BBR in previous studies.42–44 In our recent study, OBB has been observed to exhibit superior bioactivities with more favorable safety profile.19,45,46 Furthermore, OBB has been found to exist in the whole blood in the Hb-bound form in rats after intravenous injection with BBR.13 In this follow-up study, we further endeavored to investigate the conversion rate of BBR into OBB and the erythrocytes/plasma partition coefficient, an important parameter for drug distribution and the physiological interpretation. The result obtained indicated that more than 80% of BBR underwent rapid oxidation in erythrocytes with only a very small portion (8.61%) of BBR remaining in plasma in the first half-hour. When the prodrug BBR was bio-transformed to its oxidized congener OBB, the C-8 quaternary ammonium structure was converted to a more active lactam ring. The lipophilicity and absorbance of OBB would expectedly be elevated for easier ferry through biological membranes, which potentially resulted in the enhancement of its bioactivity.
In this study, OBB exhibited similar anti-inflammatory effect as BBR at an approximately 10-fold lower dose (0.1 μM). TNF-α and IL-6 potentially act as mediators of precise control and induction of inflammation, immune response and viral infection.47,48 Besides, IL-18 is also positively related to systemic inflammation and liver injury.49,50 They are the common proinflammatory cytokines which are released mainly by the hepatic macrophages/Kupffer cells via the activation of the inflammasome. The anti-inflammatory effects of OBB and BBR were potentially associated with inhibition of pro-inflammatory factors (TNF-α, IL-6 and IL-18) and inflammatory response. The pharmacokinetic studies showed that the level of OBB in vivo was more than 0.1 μM, which was sufficient to exert appreciable anti-inflammatory effect. The acetic acid-induced vascular permeability assay is a renowned model for evaluating inflammatory processes. Acetic acid intensifies various mediators (histamine, prostaglandins and 5-hydroxytryptamine) in the peritoneal cavity, causing dilation of capillaries and increasing vascular permeability.51 Our result indicated that OBB exhibited a significant inhibitory effect in a dose-dependent manner on vascular permeability in mice. Of note, the dose of OBB (approximately one-quarter of BBR dose) that showed efficacy was much less than that of BBR. This correlation observed indicated that the anti-inflammatory effect of BBR might be attributed, at least partially, to its active metabolite OBB.
Besides loading the drugs and metabolizing them into the more active metabolite, targeted delivery of these molecules to specific organs is an important feature of erythrocytes.52 To effectively achieve in situ loading of circulating drugs for therapy, the intrinsic phagocytic behavior of monocytes/macrophages is utilized to specifically engulf some endogenous substances in blood stream.53 Natural damaging/aging erythrocytes, which are regarded as blood waste materials, are specifically phagocytized and eliminated by monocytes/macrophages. This process is called erythrophagocytosis.54 Promisingly, the artificially damaging/senescent erythrocytes can also mimic the natural morphological, immunological and biochemical properties of damaged/aging erythrocytes and could be exploited to hitchhik on circulating monocytes/macrophages for the accumulation of drugs.
Autogenous drug-loaded erythrocytes, which are deemed as the damaged cells, deplete monocytes/macrophages by inducing apoptosis in the monocytes/macrophages that engulf them.55,56 It has been covered that engineered erythrocytes were employed as vehicles to deliver target drugs to the macrophages.55,57,58 For example, to optimize the bioavailability and selectivity of the insoluble drugs to active phagocytes and other components of the immune system in the mononuclear phagocyte systems (MPS), the delivery of erythrocytes-loaded anti-inflammatory drugs (eg, glucocorticoids) to pro-inflammatory cells (such as macrophages) has been conducted.59 In the present study, it was interesting to note that there was a strong positive linear correlation between drug concentration in erythrocytes and monocyte depletion. By increasing the intracellular drug concentration in erythrocytes, the ability of drug-loaded erythrocytes to promote macrophage phagocytosis increased as well, which might cause a decrease in monocytes number in the peripheral blood.
Tissue distribution of BBR and OBB, particularly in certain target organs and the active sites in vivo, could also serve as evidence of drug-loading erythrocytes targeting macrophages. In our study, dominant tissue distribution of BBR and OBB was observed in the liver. The delivery of BBR to circulating monocytes/macrophages encountered the phagocytic cells of the liver (mainly Kupffer cells). The drug-loaded erythrocytes were naturally scavenged by hepatic reticuloendothelial system (RES) macrophages (Kupffer cells), subsequently broken down in the lysosome, and caused the release and accumulation of drugs in the tissues rich in macrophages (such as liver).41,55,56,60
In theory, the spleen, the biggest immunity organ and the center of cellular immunity and humor immunity, contains numerous macrophages61 and is considered the highest uptake organ of BBR. However, the concentration of BBR in the spleen was the lowest in comparison to the other tissues. The reason behind this phenomenon might be associated with the physiology of the liver. Indeed, the liver is mainly supplied by the portal vein which transports the blood from the mesenteric venous. Therefore, drug-loaded erythrocytes reach mostly the liver where they are engulfed by Kupffer cells after intragastric administration. Such blood, poor in drug-loaded erythrocytes with the hepatic first-pass effect, subsequently flows to the other tissues through hepatic vein and then enters the blood circulation. Therefore, fewer drug-loaded erythrocytes are available to deplete macrophages of organs except for the liver. The pharmacokinetic characteristics of BBR observed and drug-delivery through phagocytosis in the RES mediated by erythrocytes might result in minimization of drug-mediated toxic effects upon nonphagocytic cells with favorable safety profile in vivo.
As summarized in Figure 12, in this paper, we indicated 1) that BBR was largely present in erythrocytes in vivo after oral administration of PCC, 2) that BBR entered erythrocytes and was metabolized to OBB after intraperitoneal injection of BBR in rats, 3) that a negative correlation was observed between total concentrations of BBR and OBB in erythrocytes and monocyte depletion in peripheral blood, and 4) that OBB exhibited similar anti-inflammatory activity as BBR with a much lower dosage in vivo and in vitro.
 |
Figure 12 Summary scheme of the scenario for the pharmacokinetics and anti-inflammatory pharmacodynamics of BBR and its active metabolite OBB from the perspective of BBR-erythrocytes self-assembly delivery system. |
Taken together, erythrocytes might unintentionally represent the carrier critically participating in biodistribution, pharmacokinetics, metabolism and target delivery of BBR/OBB into phagocytic cells. The results might interpret the mystery of the extremely low plasma concentration of BBR, and reveal the hidden and critical role of erythrocytes as the simultaneous metabolic reservoir and targeted vehicle for metabolizing and circulating BBR. The present study provided evidence to support that the anti-inflammatory activity of BBR and PCC was attributable, to a large extent, to the unique and indispensable roles of erythrocytes. It provides a novel dimension to explain the paradox between the poor bioavailability and high efficacy of some low-soluble drugs. The natural erythrocytes self-assembling delivery system might serve a non-negligible role in exploring the pharmacokinetics–pharmacodynamics relationship of natural products.
Conclusion
In conclusion, BBR was prodominantly found in erythrocytes, which critically participated in the biodistribution, pharmacokinetics, metabolism and target delivery of BBR and its metabolite. The anti-inflammatory activity of BBR and PCC was intimately associated with the metabolism into the active congener OBB and the targeted delivery to monocytes/macrophages mediated by the erythrocytes.
Abbreviations
BBR, berberine; ELISA, enzyme-linked immunosorbent assay; Hb, hemoglobin; HPLC, high-performance liquid chromatography; IL-18, interleukin-18; IL-6, interleukin-6; LPS, lipopolysaccharide; OBB, oxyberberine; PCC, Phellodendri Chinensis Cortex; RES, reticuloendothelial system; TNF-α, tumor necrosis factor alpha.
Acknowledgments
This research was financed by grants from National Science Foundation of China (Grant Nos. 82074082 and 82104472), Guangdong Natural Science Foundation (Grant Nos. 2021A1515011490 & 2022A1515011706 & 2019A1515010819), Key-Area Research and Development Program of Guangdong Province (Grant No. 2021B1515140003), and “Double First-Class” and High-level University Discipline Collaborative Innovation Team Project of Guangzhou University of Chinese Medicine (Grant 2021xk48).
Author Contributions
All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.
Disclosure
The authors declared that there was no potential conflict of interest.
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